Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (20): 3625-3628.doi: 10.3969/j.issn.1673-8225.2011.20.004

Previous Articles     Next Articles

Stress absence effect on ERK42/44 expression and intervention of Tongluo Shenggu capsules

Liu Shao-jun1, Zhang Xian2, Feng Li-min2, Chen Xiao-jun1   

  1. 1First Department of Orthopedics, First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou  510407, Guangdong Province, China
    2Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou  510006, Guangdong Province, China
  • Received:2010-11-02 Revised:2010-12-29 Online:2011-05-14 Published:2011-05-14
  • Contact: Liu Shao-jun, First Department of Orthopedics, First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510407, Guangdong Province, China
  • About author:Liu Shao-jun☆, Doctor, Associate cheif physician, Master’s supervisor, First Department of Orthopedics, First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510407, Guangdong Province, China lioshaojun@163.net
  • Supported by:

    the Science and Technolgy Commission of Guangdong Province, No. 2007B031407010*

PDF

286

Abstract:

BACKGROUND: Stress absence induces osteoporosis in bedridden patients or astronauts. Tongluo Shenggu capsules can promote bone formation, which may affect stress absence-induced osteoporosis.
OBJECTIVE: To investigate influence of stress absence on the expression of ERK42/44, and intervention of the Tongluo Shenggu capsules.
METHODS: Totally 40 SD rats were randomly divided into normal control, model and high- and low-drug groups. Exception rats in the normal control group, all animals in other three groups were subjected to tail suspension test for preparing for stress absence models. Animals in the drug groups were administered Tongluo Shenggu capsule (0.6 or1.2 g/kg) once per day. After model preparation, animals were sacrificed, and bones of lower leg was taken out. Bone The norphological changes and expression of ERK was determined by western blot.
RESULTS AND CONCLUSION: Stress absence induced osteoporosis and decreased the amount of phosphorylation ERK, but had little effect on the total ERK. Low-dose of Tongluo Shenggu capsules decreased the expression of ERK phosphorylation, but high-dose of drugs had marginal on ERK. Stress absence induced osteoporosis by decreasing the phosphorylation of ERK, and the bone formation improving effect of Tongluo Shenggu capsules is not by regulating ERK expression.

CLC Number: