Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3361-3364.doi: 10.3969/j.issn.1673-8225.2011.18.032

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Effect of oxygen free radicals on adenosine triphosphatase and somatostatin in reproductive cells

Hu Jian-xin1, He Jian1, Liu Jun1, Sun Zhao-lin1, Wang Yuan-lin1, Liu Xiu-heng2   

  1. 1Department of Urology, Guizhou Provincial People’s Hospital, Guiyang  550002, Guizhou Province, China
    2Department of Urology, Renmin Hospital of Wuhan University, Wuhan  430064, Hubei Province, China
  • Received:2010-11-19 Revised:2011-02-14 Online:2011-04-30 Published:2011-04-30
  • Contact: He Jian, Chief physician, Master’s supervisor, Department of Urology, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China gzhejian@yahoo. com.cn
  • About author:Hu Jian-xin★, Master, Associate chief physician, Department of Urology, Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China hjx918@163.com
  • Supported by:

    the Science and Technology Foundation of Guizhou Health Committee, No. gzwkj2009-1-055*

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Abstract:

BACKGROUND: Most of studies about oxygen free radicals focus on production and scavenging of free radicals in animal cells.
OBJECTIVE: To investigate the effect of oxygen free radical on adenosine triphosphatase (ATPase) and somatostatin (SOM) in spermatogonial cells cultured in vitro.
METHODS: Spermatogonial cells isolated from mice were purified and cultured in vitro and divided into two groups after 24-hour culture. In the experimental group, xanthine-xanthinoxidase (X-XO) was used to establish an oxygen free radical damaging model. In the control group, normal saline was added. After 24 and 48 hours, changes of ATPase and SOM were observed in the two groups. Gray value and absorbance value at 492 nm were determined.
RESULTS AND CONCLUSION: After 24 and 48 hours, the gray values of ATPase and SOM in the experimental group were significantly higher than those in the control group (P < 0.01), while the absorbance values in the experimental group were lower than those in the control group (P < 0.01). These indicated that oxygen free radicals can decrease the ATPase and SOM, and influence the growth and proliferation of spermatogonial cells cultured in vitro.

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