Abstract:

BACKGROUND: The application of biodegradable materials is the important research component of small-caliber tissue engineered vessels in vitro. How to modify degradable materials so as to facilitate in vitro anticoagulant and promote endothelial cell adhesion, is one of the hotspots in the study of vascular tissue engineering. 
OBJECTIVE: To in vitro load basic fibroblast growth factor (bFGF) by using biodegradable polycaprolactone (PCL) graft heparin materials, and to observe the effects on endothelial cell adhesion.
METHODS: Applying PCL biodegradable materials, the activated heparin esterified with PCL hydroxyl end and then anchored at both ends of the PCL. The vascular scaffold was prepared with electrospinning technique. Using the electrostatic adsorption between heparin and growth factors, the scaffold was loaded with bFGF. After low-density short-term static cultivation of endothelial cells, the bFGF-loaded biodegradable PCL on the adhesion of endothelial cells was investigated.
RESULTS AND CONCLUSION: The bFGF-loaded biodegradable PCL graft heparin scaffold is successfully fabricated. Endothelial adhesion experiments demonstrated that the biodegradable scaffold was conducive to endothelial cell adhesion. Biodegradable PCL graft heparin material loading bFGF is beneficial to endothelial cell adhesion in vitro.