Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1341-1344.doi: 10.3969/j.issn.1673-8225.2011.08.003

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Blood flow and vessel wall of heterogeneous animals transplanted with tissue engineered small vessel scaffolds

Chen Hua-yong1, Bai Shu-ling2, Cui Chun-lan1   

  1. 1Department of Human Anatomy, Preclinical Medicine College of Yanbian University, Yanji   133000, Jilin Province, China
    2Department of Tissue Engineering, China Medical University, Shenyang   110001, Liaoning Province, China
  • Received:2010-09-18 Revised:2010-10-23 Online:2011-02-19 Published:2011-02-19
  • Contact: Bai Shu-ling, Doctor, Professor, Department of Tissue Engineering, China Medical University, Shenyang 110001, Liaoning Province, China baishuling@hotmail.com
  • About author:Chen Hua-yong☆, Doctor, Associate professor, Master’s supervisor, Department of Human Anatomy, Preclinical Medicine College of Yanbian University, Yanji 133000, Jilin Province, China chenhy6039@hotmail.com
  • Supported by:

    a grant from Jilin Provincial Educational Commission, No. 01002*; Doctor Priming Fund of Yanbian University, No. 200892*

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Abstract:

BACKGROUND: The heterogenic antigen has been tentatively removed from acellular heterogeneous small vessel scaffolds due to inducing transplant rejection.
OBJECTIVE: To transplant acellular tissue of Wistar rat’s caudal artery into blood vessel of Japanese white rabbit’s ear, and to discuss the changes of blood flow and vessel wall after the transplantation.
METHODS: Fifteen Wistar rats were collected as donors, two caudal truncus arteriosus (2.50 cm long each) were harvested from each donor, then thirty caudal truncus arteriosus served as heterogeneous small vessels and were randomly assigned into two groups,15 of which as the caudal artery group, while the remaining were treated with 1% Triton X-100 as the small vessel scaffold group for tissue engineering. Fifteen Japanese white rabbits were involved as receptors, we took central arteries from left and right ears of each receptor. Under surgery microscope, the proximal broken end of Japanese white rabbit’s central artery was totally overlapped into the proximal lumen of donor’s vessel, while the distal broken end was sewed up by general end-to-end anastomosis. Blood flow in vessel wall was continuously observed through the staining of vascular lumen and wall.
RESULTS AND CONCLUSION: The immediate patency rate of the recombinant blood vessel was 100%.The maximum time of the blood flowing of heterogenous scaffold from vessel was 46 hours and 47 minutes, which was longer than that of small blood vessel transplantation (14 hours). The poor blood flow primarily occurred in end-to-end anastomosis from the distal broken; in the overlapping, the pedicle graft structures were connected between the small vessel scaffold and the central artery tunica. On day 10, tunica intima fibers, arranged in cylindrical conformation, were seen in the small vessel scaffold, with no cell attachment. On day 100, heterogenous scaffold form vessel still kept its shape and structure. Acellular heterogenous small vessel scaffold as a vascular graft can anastomose in animal hosts, the overlapping anastomosis is superior to the end-to-end anastomosis method.

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