Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1345-1349.doi: 10.3969/j.issn.1673-8225.2011.08.004

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Guinea pig bone marrow-derived mesenchymal stem cells combined with nano-hydroxyapatite in constructing tissue-engineered artificial otosteon in vitro

Shi Xiao-lu1, Liu Li-zhong2, Xie Feng-lian3, Wang Lu-xiang4   

  1. 1First Clinical Hospital of Xinjiang Medical University, Urumqi   830054, Xinjiang Uygur Autonomous Region, China
    2First Affiliated Hospital of Xinjiang Medical University, Urumqi   830054, Xinjiang Uygur Autonomous Region, China
    3Xinjiang Medical University, Urumqi   830054, Xinjiang Uygur Autonomous Region, China
    4Xinjiang University, Urumqi   830000, Xinjiang Uygur Autonomous Region, China
  • Received:2010-09-13 Revised:2010-10-12 Online:2011-02-19 Published:2011-02-19
  • Contact: Liu Li-zhong, Doctor, Chief physician, Master’s supervisor, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China liulizhongno.1@163.com
  • About author:Shi Xiao-lu★, Studying for master’s degree, First Clinical Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China lauren84103@sina.com
  • Supported by:

    Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 200821150*

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Abstract:

BACKGROUND: Artificially synthetic nano-hydroxyapatite (nHA) has good biocompatibility and bioactivity. It can tightly connect with in vivo tissues closely within a short time after implanted into the body. Therefore nHA has been widely used for bone tissue engineering.
OBJECTIVE: To observe the compounded culture degree of guinea pig bone marrow-derived mesenchymal stem cells (MSCs) and nHA, to analyze the feasibility of constructing tissue-engineered artificial otosteon.
METHODS: The MSCs were isolated from the bone marrow of guinea pig by differential adhesion method. The surface markers of MSCs were examined CD29, CD44, CD45 by flow cytometric analyses. The differentiated capability of MSCs was also detected. The MSCs were cultured with nHA porous ceramic, and their adhesion on the scaffold was observed by electron microscope at 1, 3, 5, 7, 10 days.
RESULTS AND CONCLUSION: After three days cultivation, a large amount of MSCs attached on the material surface with stable form, proliferation activity and terrific extensibility; Composite material had all covered MSCs at 5 days, cell surface had a large number of secretary granules, soma amplified obviously, edge of cells was complete and fibroid extension; 7 days later cells gradually fell from the material surface, still attached to the material surface and appeared "star" or "pseudopods"; 10 days later, cells flaked off the material surface. It can be explained that nHA maintains a good biocompatibility, which is beneficial for MSCs adhesion, proliferation and combination, nHA can be used to construct tissue-engineered artificial otosteon.

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