Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (7): 1245-1248.doi: 10.3969/j.issn.1673-8225.2011.07.023

Previous Articles     Next Articles

Expression of Bcl-xL gene in normal skin and the proliferating and degenerating hemangioma tissues

You Hao1, Gao Jin-song2, Chen Fang-zhou1, Zhu Sheng-wen1, Wu Lei1   

  1. 1Department of Orthopedics, Second Affiliated Hospital of Jianghan University, Wuhan Fifth Hospital, Wuhan  430050, Hubei Province, China
    2Department of Basic Medicine,  Changsha Medical College, Changsha  410219, Hunan Province, China
  • Received:2010-09-05 Revised:2010-12-09 Online:2011-02-12 Published:2011-02-12
  • About author:You Hao★, Master, Associate chief physician, Department of Orthopedics, Second Affiliated Hospital of Jianghan University, Wuhan Fifth Hospital, Wuhan 430050, Hubei Province, China yourenyouyu2010@yahoo.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30271345*; the Science and Technology Department of Hubei Province, No. 2004AA301c107*; the Health Department of Hubei Province, No. JXIB075*

PDF

370

Abstract:

BACKGROUND: Bcl-xl gene can suppress cell apoptosis and may participate in angiopoiesis. However, more studies concerning Bcl-xL gene in malignant tumor, few are reported in hemangioma. 
OBJECTIVE: To study the expression of Bcl-xL gene in normal skin vessels, hyperplasia and degeneration hemangioma tissues using immune-histochemical technique.
METHODS: Specimens were obtained from 40 cases of dermal hemangioma by exairesis, comprising 22 cases with proliferating hemangiomas and 18 with degenerating hemangiomas. In addition, normal skin tissues around hemangiomas from 5 cases were also chosen for controls. Immunohistochemical stainings were performed to detect the expression of Bcl-xL in those three groups. Endothelial cells were identified by expressing factor Ⅷ-related antigen. The average absorbance and the rate of positive area of expression of Bcl-xL were analyzed.
RESULTS AND CONCLUSION: There were plenty of brown particles in the cytoplasm of proliferating endothelial cells, which strongly positive expressed Bcl-xL. There were few and mo brown particles in endothelial cells cytoplasm of the degenerating hemangiomas or normal skins. The expression of Bcl-xL were weakly and no expression. Compared with the normal skins, the average absorbance and rate of Bcl-xL positive expression were higher in the proliferative and degenerating hemangiomas (P < 0.01). Bcl-xL is an anti-apoptosis gene and was highly expressed in the proliferative phase, which promoted proliferation of blood vessel endothelial cells and participated in hyperplasia of hemangiomas.

CLC Number: