Abstract:

BACKGROUND: The biological surface modification for the titanium and improving the biocompatibility of titanium surface is one of the hot spots in research of titanium surface modification.
OBJECTIVE: Collagen and heparin multilayers were coated on pure titanium using a layer-by-layer self-assembly technique, to achieve extracellular matrix modification of the titanium surface and to improve the cytocompatibility of titanium.
METHODS: The film growth was initialized by depositing one layer of positively charged poly-L-lysine on the NaOH-treated titanium substrate (porous and negatively charged surface). Then, the film was formed by the alternate deposition of negatively charged heparin and positively charged chitosan via electrostatic interactions of polyelectrolytes, and terminated with an outermost layer of collagen. The chemical composition, surface topography as well as roughness of the multilayers were investigated by using diffuse reflection-fourier transform infrared spectroscopy, scanning electron microscope, atomic force microscope, and contact angle measurement respectively. We cultured bone marrow stromal cells on the surface of modified-titanium and pure titanium respectively to observe its adhesion and proliferation.
RESULTS AND CONCLUSION: The analysis of diffuse reflection-fourier transform infrared spectroscopy, scanning electron microscope, atomic force microscope, and contact angle measurement confirmed that, heparin-collagen multilayers formed gradually on the titanium surface and could improve cell adhesion, proliferation and differentiation. The heparin-collagen multilayers is a potential biological titanium surface to improve the biocompatibility of titanium surface.