Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9591-9594.doi: 10.3969/j.issn.1673-8225.2010.51.020

Previous Articles     Next Articles

Preparation and cytotoxicity of carboxymethyl chitosan thermosensitive hydrogel

Peng Wei, Mu Yu   

  1. Department of Stomatology, North China Coal Medical University, Tangshan   063000, Hebei Province, China
  • Online:2010-12-17 Published:2010-12-17
  • About author:Peng Wei★, Master, Associate professor, Associate chief physician, Master’s supervisor, Department of Stomatology, North China Coal Medical University, Tangshan 063000, Hebei Province, China pengwei1968@sina.com
  • Supported by:

    Tackling Key Program of Science and Technology in Hebei Province, No. 07275524*; Chinese Integrative Medicine Science and Technology Projects by Hebei Provincial Administration of Traditional Chinese Medicine in 2009, No. 2009056*

PDF

620

Abstract:

BACKGROUND: Compared with chitosan, carboxymethyl chitosan is safe, nontoxic, antigenic-free, and has higher water-solubility, membrane-forming, moisture-retention capacity and good biocompatibility, it is a good drug carrier.
OBJECTIVE: To produce carboxymethyl chitosan thermosensitive hydrogel, and to evaluate its cytotoxicity on mouse lung fibroblasts (L929).
METHODS: The carboxymethyl chitosan mixed with glycerophosphate salt to produce the carboxymethyl chitosan thermosensitive hydrogel. MTT colorimetric assay was used to determine the cytotoxicity of L929 cells at different concentrations (50, 10, 2, 0.4, 0.08) of carboxymethyl chitosan thermosensitive hydrogel, positive and negative control groups were also set. Absorbance values were detected at 24, 48, 72, 96 hours after adding the sample, the relative growth rates were counted and cytotoxicity grade was assessed.
RESULTS AND CONCLUSION: The relative growth rates were ranged from 103% to 228% at different time points and cytotoxicity grade of different concentrations of the carboxymethyl chitosan thermosensitive hydrogel was 0. The carboxymethyl chitosan thermosensitive hydrogel shows no cytotoxicity on L929 and has good biocompatibility, it is potential in treatment of periodontal diseases.

CLC Number: