Abstract:

BACKGROUND: Sox gene family is a newly discovered gene family, it plays an important role in the process of embryonic development, sex differentiation, development of nervous system and skeletal system.
OBJECTIVE: To investigate the biocompatibility of SOX-9 gene transfected bone marrow stroma cells (BMSCs) with poly lactic-co-glycolic acid (PLGA) complex, and the feasibility of BMSCs transfected by SOX-9 gene for repairing cartilage defect in animal models.
METHODS: BMSCs successfully transfected by SOX-9 gene were cultured on PLGA copolymer, and animal models with cartilage defect were prepared. The complex was planted into defect site of animal model. The effect of repair was evaluated by macroscopic sample observation, histological observation, collagen Ⅱ immunohistochemistry and RT-PCR.
RESULTS AND CONCLUSION: BMSCs transfected by SOX-9 gene grew on PLGA copolymer well and high expression of collagen Ⅱ was observed. The complex transplanted into defect site could repair the cartilage defects in animal model. There is well biocompatibility between PLGA copolymer the transfected BMSCs. The complex could repair the cartilage defect in rabbit models.