Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9555-9558.doi: 10.3969/j.issn.1673-8225.2010.51.012

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Preparation and preservation of tissue engineered vascular matrix

Chi Yi-fan1, Xu Hui2, Lin Ming-shan1, Hou Wen-ming1, Sun Zhong-dong1, Sun Long1, Niu Zhao-zhuo1, Sun Yong1, Sheng Wei1   

  1. 1 Department of Cardiovascular Surgery, Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao  266071, Shandong Province, China; 2 Department of Anesthesiology, Affiliated Hospital, Medical College of Qingdao University, Qingdao  266071, Shandong Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Lin Ming-shan, Master, Physician, Department of Cardiovascular Surgery, Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao 266071, Shandong Province, China Lincoln3086@yahoo.com.cn
  • About author:Chi Yi-fan☆, Doctor, Chief physician, Professor, Master’s supervisor, Department of Cardiovascular Surgery, Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao 266071, Shandong Province, China chiyf@hotmail.com
  • Supported by:

    a grant by Science and Technology Bureau of Qingdao, No. 06-2-2-6-nsh*

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Abstract:

BACKGROUND: Acellular vascular matrix is prepared and preserved in order to construct complete biological tissue engineered vessels.
OBJECTIVE: To prepare tissue engineered vascular matrix and to investigate the feasibility of preserving it in liquid nitrogen.
METHODS: Trypsin, hypertonic solutions and chemical detergent were applied for a multi-step process to prepare acellular tissue engineered vascular matrix from rabbit thoracic aorta. The specimen was observed with macroscopic observation, light microscope, scanning electron microscope and transmission electron microscope. Then the specimen was observed by scanning electron microscope after preserved in liquid nitrogen for 3 months.
RESULTS AND CONCLUSION: The cells were removed completely after this processing in rabbit blood vessels, the elastic fibers and collagen fibers were preserved well, extracellular matrix maintained intact. There were no obvious differences in scanning electron microscopy observation between the specimen preserved in liquid nitrogen for 3 months and the fresh matrix. A combined method of trypsin, hypertonic solution and chemical detergent can be a better method to prepare tissue engineered vascular matrix, and the vascular matrix could be preserved in liquid nitrogen for a short time.

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