Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9512-9516.doi: 10.3969/j.issn.1673-8225.2010.51.003

Previous Articles     Next Articles

Tissue engineered characterization of allogenic demineralized bone matrix

Feng Wan-wen1, Xia Ya-yi2, Dang Yue-xiu2, Yang Guang-zhi1, Li Guang-mei1, Li Xiao-min1, Chai Ling-xia1, Chang Ling-wen1,  Zhai De-zhong1, Cui Jian-guo1, Wang Xiao-hong1, Wu Ru1, Li Ting1, Guo Kan-suo1   

  1. 1 Department of Orthopeadics, East Hospital of Lianyungang, Xuzhou Medical College, Lianyungang Hospital for Foreigners, Lianyungang   222042, Jiangsu Province, China; 2 Department of Orthopeadics, Second Hospital of Lanzhou University, Lanzhou   730030, Gansu Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Cui Jian-guo, Attending physician, Department of Orthopeadics, East Hospital of Lianyungang, Xuzhou Medical College, Lianyungang Hospital for Foreigners, Lianyungang 222042, Jiangsu Province, China cjglyg@126.com
  • About author:Feng Wan-wen★, Master, Associate chief physician, Department of Orthopeadics, East Hospital of Lianyungang, Xuzhou Medical College, Lianyungang Hospital for Foreigners, Lianyungang 222042, Jiangsu Province, China wwfeng1968@sohu.com
  • Supported by:

    a grant by Lianyungang Bureau of Health, No. 09014*

Abstract:

BACKGROUND: Scaffold materials derived from bone have incomparable advantages compared with synthesized materials, regarding morphological or mechanical characterization. Demineralized bone matrix represents three-dimensional structure proximal to autogenous bone, it mainly contains Ⅰ-type collagen that is a good scaffold for cells adhesion and growth.
OBJECTIVE: To study the biological characteristics of allogenic demineralized bone matrix (DBM) as viewed from tissue engineering, and to investigate the feasibility of DBM as a scaffold of cartilage tissue engineering.
METHODS: DBM was prepared from chinchilla rabbits according to the methods described by Urist. Ultrastructures of DBM were observed by scanning electron microscope, the pore diameter, porosity and degradable rate of DBM were determined. Adhesive rate of bone marrow-derived mesenchymal stem cells into allogenic DBM was detected. Histocompatibility of allogenic DBM was evaluated by means of DBM implanting in rabbits.
RESULTS AND CONCLUSION: DBM represented three-dimensional spongy-like structure with pores. Pore diameter of DBM ranged from 210 μm to 320 μm, mean porosity was 92%. Degradable rate of DBM in vitro at 12 weeks achieved over 90%. Adhesive rate of bone marrow-derived mesenchymal stem cells into allogenic DBM were (51.50±2.30)%, (94.13±2.14)% and (87.24±1.75)% at 2, 4 and 6 days respectively after coculture. After allogenic DBM were implanted in rabbits at 6 weeks, interface between DBM and muscle tissue had no evident signs of inflammatory and rejective reaction, cartilage-like and bone tissue were also formed. DBM represents a three-dimensional porous structure which fits for adhesion and proliferation of seeding cells. Biodegradation of DBM synchronizes cartilage formation in phase. Allogenic DBM shows excellent adhesive activity and biocompatibility with cells, it can satisfy the requests of cartilage tissue engineering for scaffold materials, thus considered as an ideal scaffold material for cartilage tissue engineering.

CLC Number: