Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (51): 9517-9521.doi: 10.3969/j.issn.1673-8225.2010.51.004

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Effects of strontium ranelate on osteocyte apoptosis in rats under simulated weightlessness

Jian Lian1, Huang Zhen2, Mai Yan-xing1, Wang Mai-tao1, Su Jia-lin1, Deng Wei-min3   

  1. 1 Department of Endocrinology, 2 Department of Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou   510282, Guangdong Province, China; 3 Department of Overseas Chinese, Guangzhou General Hospital of Guangzhou Military Area Command of Chinese PLA, Guangzhou   510282, Guangzhou Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Huang Zhen, Doctor, Professor, Master’s supervisor, Department of Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China hzhen2@sina.com
  • About author:Jian Lian★, Studying for master’s degree, Department of Endocrinology, Zhujiang Hospital, southern Medical University, Guangzhou 510282, Guangdong Province, China jianlian_ky@163.com

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Abstract:

BACKGROUND: Simulated weightlessness promotes osteoblast and osteocyte apoptosis, and strontium can reduce the apoptosis rate of osteoblasts and osteocytes in bone tissues under weightlessness, and promote bone formation.
OBJECTIVE: To investigate the prevention and therapeutic effect of strontium ranelate on cells apoptosis under weightlessness state.
METHODS: SD rats aged 5 weeks were included in this study to establish weightlessness models, intragastrically administrating strontium ranelate 3 days before suspension or while suspension. At 7 days after model establishment, serum calcium, phosphorus, alkaline phosphatase levels were observed by using automatic biochemistry analyzer. The bone Gla protein concentration was determined by radio immunoassay. The cell apoptosis in bone tissues was detected with in situ cell apoptosis detection technology. The expressions of Fas protein in bone tissues were detected with SP immunohistochemical method.
RESULTS AND CONCLUSION: In the model rats of simulated weightlessness, serum bone Gla protein and alkaline phosphatase contents were significantly lower than those in corresponding control group (P < 0.05), whereas serum calcium and phosphorus concentrations were higher than that in control group (P < 0.01), the apoptosis of osteoblasts, osteocytes and bone marrow mesenchymal cells, as well as Fas antigen in bone tissues were all increased compared with control group (P < 0.01). Serum bone Gla protein and alkaline phosphatase content in rats treated with strontium ranelate 3 days before or while suspension were significantly higher than that in model group (P < 0.05), whereas serum calcium, cell apoptosis and Fas expression in bone tissues were significantly lower than model group (P < 0.05). Tail suspension stimulated weightlessness in rats can induce an increase of osteoblasts, osteocytes and bone marrow cells apoptosis associated with increased Fas expression in bone tissue, whereas strontium ranelate decreased Fas expression, accordingly, decreased osteoblasts, osteocytes and bone marrow cells apoptosis.

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