Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (50): 9378-.doi: 10.3969/j.issn.1673-8225.2010.50.017

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Analysis of gene expression difference following spinal cord injury in rats using complementary DNA microarray

Qiang Hua1, Zhou Yan2, Liu Jin2, Chen Xiong-shen3, Huang Xiao-wei2, Zhang Bin2, Yin Bin2, Yuan Jian-gang2, Jia Lian-shun3   

  1. 1 Department of Orthopaedics, Beijing Tongren Hospital, Capital Medical University, Beijing  100730, China; 2 Department of Biochemistry and Molecular Biology, Basic Research Institute, Chinese Academy of Medical Sciences, Beijing  100005, China; 3 Institute of Spinal Surgery, Changzheng Hospital, the Second Military Medical University of Chinese PLA, Shanghai  200003, China
  • Online:2010-12-10 Published:2010-12-10
  • About author:Qiang Hua☆, Doctor, Associate chief physician, Department of Orthopaedics, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China Qhxq_cn@sina.com

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Abstract:

BACKGROUND: Gene microarray can be used to parallel detect expression patterns of large amounts of genes, which overcome the limitation of traditional only single or multiple genes detection. 
OBJECTIVE: To dynamic observe the changes of gene expression in rat acute spinal cord injury (SCI) models using complementary DNA microarray consisting 1 176 genes.
METHODS: Seventy female SD rats were randomly divided into the normal control, surgery control, 4-, 24-hour and 3-, 7-, 10-day injury groups. Rats in the injury groups subjected to T7 and T8 excision, and made SCI models by high falling. Sham animals received only a laminectomy. T6-10 spinal cord was harvested at each time point, and autoradiographic gene expression profile was analyzed by AtlasImageTM 2.01 software (Clontech). Compared with the normal group, greater than 3-fold changes considered as differential expression.
RESULTS AND CONCLUSION: We identified 81 genes that showed a greater than 3-fold change in SCI tissues, including 46 genes up regulated and 35 genes down regulated. In addition, changes of neurokinin B, neuropeptide Y and postlobin-v2 receptor gene were firstly found during SCI. The results demonstrated that, gene expression profile during acute SCI can be observed by using gene microarray combined with experimental animal models, which has significance for further pathogenesis study at gene levels.

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