Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (47): 8773-8776.doi: 10.3969/j.issn.1673-8225.2010.47.008

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Vertebral body defect augmentation with silk fibroin/calcium phosphate cement in vitro

Chen Xiao-qing1, Zhao Jian1, Zhang Feng1, Wang Gen-lin2, Gan Min-feng2, Gu Yong2, Zhu Xue-song2, Yang Hui-lin2   

  1. 1 Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong   226001, Jiangsu Province, China; 2 Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou   215006, Jiangsu Province, China
  • Online:2010-11-19 Published:2010-11-19
  • Contact: Yang Hui-lin, Professor, Chief physician, Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China osteogen@gmail.com
  • About author:Chen Xiao-qing☆, Doctor, Physician, Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China chenxiaoqing1981@gmail.com

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Abstract:

BACKGROUND: Injection into the vertebral fracture model of corpse is the most common used method for screening bone cement biomechanically in vertebral augmentation. This model can hardly be applied in the further studies in vivo.
OBJECTIVE: To develop a vertebral body defect model for screening vertebral augmentation filler materials and to evaluate the mechanical property after the bone cement augmentation.
METHODS: Forty-eight lumbar vertebrae harvested from ten fresh skeletally mature female sheep cadavers were evenly assigned to eight groups, 6 vertebrae in each. Four groups of vertebrae were drilled in the perpendicular direction to center of the vertebra, with the different drills at a diameter of 2.0, 4.0, 6.0, 8.0, to form a cylindrical bone defect. All vertebrae were compressed to determine the strength and stiffness, which were compared with normal vertebrae group. Based on the biomechanical test, a certain defect was selected as the vertebral body defect model. The remaining 3 groups were prepared into bone defect models, and then augmented by calcium phosphate cement, silk fibroin/calcium phosphate cement, polymethyl methacrylate, respectively. The biomechanical test was performed after setting for 24 hours in simulated body fluid.
RESULTS AND CONCLUSION: The compressive strength and stiffness of the vertebrae with bone defect gradually decreased as the diameter of the bone defect increased, and the decline was remarkable in the vertebrae at a diameter of 6.0 mm. After bone defect in vertebrae was produced using a 6.0-mm-diameter drill, there was no difference in the compressive strength and stiffness of the vertebrae among silk fibroin/calcium phosphate cement, polymethyl methacrylate compared with normal vertebrae group (P > 0.05), while calcium phosphate cement group had a lower value compared with normal vertebrae group (P < 0.05). The sheep vertebra with a 6.0-mm diameter and 10.0-mm depth defect is a suitable model to screen vertebral augmentation filler materials. This model keeps the vertebral figuration intact and can be used to adjust the performance of vertebral enhancer in vitro, so it can be used in further studies in vivo. Silk fibroin/calcium phosphate cement has a prompt and efficient augmentation effect in bone defect vertebral model.

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