Abstract:

BACKGROUND: Collagen is the main component of demineralized cancellous bone, because of its excellent three-dimensional space and growth factors that promote cellular proliferation and differentiation, it has a dual function of bone conduction and bone induction, but there are no amendments of a standard scientific preparation process.
OBJECTIVE: To prepare rabbit demineralized cancellous bone, to detect the porosity, degradation rate and biocompatibility, and to investigate the feasibility of demineralized cancellous bone used for tissue engineering scaffolds.
METHODS: Based on Urist’s method, demineralized cancellous bone was prepared using a new method, and the porosity, in vitro degradation rate, cell viability and ossification ability of inducing bone marrow mesenchymal stem cells were determined. The experiment was divided into three groups at decalcification 3, 24, 48 hours. The other processing steps were the same.
RESULTS AND CONCLUSION: The porosity of decalcification 3, 24, 48 hours was 76.56%, 81.25%, 84.38%; complete degradation time was 64, 59, 53 days, respectively. Cells in 1-3 days after incubation were latent phase, entered the logarithmic growth phase at 5 days, then entered the stagnate phase. The induced bone marrow mesenchymal stem cells were stained positive for alkaline phosphatase. The demineralized cancellous bone prepared by new methods has no toxicity and can meet the biological characteristics requirements of bone tissue engineering scaffold materials.