Abstract:

BACKGROUND: Previous studies have shown that decellularized vascular matrix prepared is suitable for the growth of smooth muscle cells and endothelial progenitor cells, but the effect of cell inoculation density on cell coverage of vascular matrix in co-culture model remains unclear.
OBJECTIVE: To observe the growth of seeding cells at different densities implanted on decellularized vascular matrix.
METHODS: Cell implantation was performed using two-step seeding method. Firstly, smooth muscle cells were seeded onto the decellularized vascular matrix with different densities, and cultured for 3 days, and then endothelial progenitor cells were then seeded onto the smooth muscle cells-vascular matrix compound with different densities, to construct lamellar tissue engineered materials. The samples were harvested at 3 days and 1 week after the implantation of endothelial progenitor cells, cell growth was observed by scanning electron microscope.
RESULTS AND CONCLUSION: Smooth muscle cells and endothelial progenitor cells grew well on the decellularized vascular matrix, cells arranged differently on the matrix with different seeding densities; with the increase of seeding density, cells showed higher covering rate on the material surface. Tissue engineering blood vessels can be constructed by implanting suitable density of seeding cells to the decellularized vascular matrix using a two-step method.