Abstract:

BACKGROUND: Previously, whether the brain ischemia is exist or not judged by positive syndrome, which would be influenced artificially, and the target is also not strictly. The tannic acid-ferric chloride method is characterized by reliable, simple and reproducible. Accordingly, this method can make it possible for the scientific researcher to overcome the above deficiencies by screening cerebral artery circle of Willis of Mongolian gerbils.
OBJECTIVE: To explore a simple and reliable method for screening the Willis circle variation of arteries in Mongolian gerbils.
METHODS: The forebrain ischemia models of Mongolian gerbils were established by using Kirino’s method. Laser spectroscopy was used to observe the blood flow in the cerebral artery, and tannic acid-ferric chloride method was used to display cerebral microvasculars.
RESULTS AND CONCLUSION: After common carotid arteries were legend, those Mongolian gerbils, which laser spectroscopy was still able to detect the blood flow in forebrain, have circle of Willis arteries, as confirmed by tannic acid-ferric chloride method. The results demonstrated that it is quickly and reliably to screen Willis circle variation in Mongolian gerbils using tannic acid-ferric chloride method to display brain microvasculars.