Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (35): 6567-6572.doi: 10.3969/j.issn.1673-8225.2010.35.027

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Acquisition of complete locating points on 2-D Panoramagram for registration of functional fascicular groups of the peripheral nerve

Zhang Yi1, Lu Hao2, He Cai-feng3, Qi Jian4, Luo Peng4, Liu Xiao-lin4   

  1. 1 Department of Plastic and Reconstructive Surgery, 3 Orthopedic Institute, 4Department of Microsurgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China; 2 Division of Plastic Surgery, Third Military Medical University, Chongqing  400038, China
  • Online:2010-08-27 Published:2010-08-27
  • Contact: Liu Xiao-lin, Professor, Doctoral supervisor, Department of Microsurgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China gzxiaolinliu@hotmail.com
  • About author:Zhang Yi☆, Doctor, Attending physician, Department of Plastic and Reconstructive Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China Doczy2006@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30571913*; the Natural Science Foundation of Guangdong Province, No. 91510089010000 06*

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Abstract:

BACKGROUND: Computer automatic registration of 2-D images is a key step for 3-D reconstruction. The software development of nerve cross section requires 4 complete locating points.
OBJECTIVE: To explore the key technology to capture the complete locating points on 2-D Panoramagram for registration of the functional fascicular 3-D visualization of peripheral nerve.
METHODS: A segment donating fresh adult sciatic nerve with 4 piece of woman’s hairs fixed around the nerve was serially horizontally sliced into 100 slices, 10 µm thick. According to the different adhesion glass (polylysine coated silicification glass and ordinary glass) and the different ways of staining (hematoxylin-eosin and Karnovsky-Roots AChE staining). The 2-D panorama images of nerve section slice before and after staining were obtained by OLYMPUS-Z61 micro digital photography device. The counts of hair locating pots of each image were compared between pro and post staining. The slices of a segment donating fresh adult ulnar nerve of upper arm were stained with AchE, 2-D panorama images of the same slice were captured before and after AchE staining. Using the layer processing technique of Photoshop CS, the recomposition images including complete 4 location pots were obtained.
RESULTS AND CONCLUSION: The missing of hair locating pots was serious after staining. The remaining hair location points on the glass had no significant differences among the groups (P > 0.05); the rate of complete adhesion of 100 image was 1%, with a total adhesion rate of 22.5%. The hair locating pots adhesion rate of frozen slices of peripheral nerve is low after staining. Staining did not affect adhesion rate: the rate of complete adhesion and the rate of total adhesion before staining were 98.0% and 99.50% respectively. The position of the pots before staining was accurate. The outline of the merging 2-D panorama images has fine goodness of fit，the 4 locating points of is accurate, the whole procedure is simple and fast. Based on the two-time imaging technique of the same slice before and after AChE staining and the photoshop layer processing technique, the 2-D panorama images with complete locating points can be obtained.

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