Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (35): 6536-6539.doi: 10.3969/j.issn.1673-8225.2010.35.019

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Application of 64-slice CT imaging in the evaluation of basic fibroblast growth factor on angiogenesis in myocardial ischemia rabbits

Long Kai-lin, Zhou Pan   

  1. Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha  410013, Hunan Province, China
  • Online:2010-08-27 Published:2010-08-27
  • About author:Long Kai-lin, Technician-in-charge, Department of Radiology, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China longkl328@126.com
  • Supported by:

    the Science and Technology Foundation of Health Department of Hunan Province, No. B2009-065*

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Abstract:

BACKGROUND: 64-slice spiral CT provides noninvasive approach that can potentially expand the utility of coronary angiography. However, there are few reports concerning 64-slice spiral CT in evaluating therapeutic effect of gene therapy.
OBJECTIVE: To evaluate the effect of basic fibroblast growth factor (bFGF) on angiogenesis in myocardial ischemia using 64-slice spiral CT.
METHODS: New Zealand rabbits with acute myocardial ischemia were randomly assigned to the bFGF group and control group. Totally 200 μL buffer phosphate containing polyamidoamine carrying bFGF cDNA nano-carriers or buffer phosphate only was injected into ischemia myocardia. Four weeks after injection, 64-slice spiral CT imaging and immunohistochemistry were applied to examine the region of ischemia myocardial and increased density of capillaries.
RESULTS AND CONCLUSION: 64-slice spiral CT imaging demonstrated that, the percentage of ischemia myocardial region in the treatment group was obviously smaller than that of the control group (P < 0.05). Furthermore, density of capillaries and arteries were increased in the myocardial section of the treatment group (P < 0.05). The findings indicated that, gene therapy with PAMAM-bFGF induces arteriogenesis in the region of myocardial ischemia. 64-slice spiral CT can evaluate therapeutic efficacy of gene therapy for myocardial ischemia.

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