Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (33): 6099-6102.doi: 10.3969/j.issn.1673-8225.2010.33.005

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Receptor activator of nuclear factor kappa B protein inhibits osteoclast activity 

Zhang Li-cheng, Zhang Li-hai, Huang Peng, Tang Pei-fu   

  1. Department of Orthopedics, General Hospital of Chinese PLA, Beijing  100853, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Tang Pei-fu, Doctor, Professor, Doctoral supervisor, Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China pftang301@126.com
  • About author:Zhang Li-cheng★, Studying for master’s degree, Physician, Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China zhanglcheng218@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30672135*; the Natural Science Foundation of Beijing, No. 7072072*

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Abstract:

BACKGROUND: Receptor activator of nuclear factor κB (RANK) plays an important role in the signaling pathways of osteoclasts differentiation and functional regulation. Therefore, it provides a new and effective method for bone resorption diseases.
OBJECTIVE: To investigate the effects of recombinant RANK protein on the activation and resorption activity of osteoclasts.
METHODS: Osteoclasts from SD rats were harvested and cultured, recombinant RANK protein with different concentrations was added into these culture systems. The growth of osteoclasts was observed by tartrate-resistant acid phosphatase and toluidine blue staining. 
RESULTS AND CONCLUSION: The number of osteoclasts was obvious decreased at 3 days after adding RANK protein, especially in the 10-4 mol/L group. The bone resorption pits were dramatically reduced at 9 days after adding RANK protein. Therefore, it suggested that recombinant RANK protein can effectively inhibit osteoclasts differentiation and resorption activity.

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