Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (33): 6095-6098.doi: 10.3969/j.issn.1673-8225.2010.33.004

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Influence of insulin-like growth factor-1 on mouse osteoblast proliferation and alkaline phosphatase activity

Guo Ling1, Wang Min2, Hao Liang2   

  1. 1 Department of Prosthodontics, Hospital of Stomatology Affiliated to Luzhou Medical College, Luzhou   646000, Sichuan Province, China; 2 Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu   610041, Sichuan Province, China
  • Online:2010-08-13 Published:2010-08-13
  • Contact: Wang Min, Professor, Doctoral supervisor, Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China hxkqwangm@163.com
  • About author:Guo Ling★, Master, Lecturer, Department of Prosthodontics, Hospital of Stomatology Affiliated to Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the Foundation of Health Department of Sichuan Province, No. 20090201* 

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Abstract:

BACKGROUND: Insulin-like growth factor-1 (IGF-1) is a growth factor to promote mitosis and regulate bone formation. It is of great significance on the dynamic process of osteoclast-driven bone resorption and osteoblast-mediated bone formation.
OBJECTIVE: To elucidate the effect of IGF-1 on proliferation of mouse osteoblasts cultured in vitro and alkaline phosphatase activities.
METHODS: Mouse osteoblasts cultured in vitro were added into medium containing various concentrations of IGF-1 (25, 50, 100, and 200 μg/L). Cell proliferation was determined using Cell Counting Kit-8 method; Cell cycle and apoptosis was assayed with flow cytometry. Alkaline phosphatase activity was examined with enzyme-linked immunosorbent assay at 3, 6 and 9 days following culture.
RESULTS AND CONCLUSION: All IGF-1 concentrations used (25-200 μg/L) were effective to promote the proliferation of mouse osteoblasts (P < 0.05), showing significant concentration-dependent effects. The maximum response was obtained with 200 μg/L, at which of IGF-1 could notably increase the percentage of cells at S phase, which indicated that IGF-1 can accelerate cellular proliferation to ensure the number of osteoblasts that participated bone rebuilding and to promote bone tissue regeneration. Simultaneously, IGF-1 at various concentrations (25-200 μg/L) could significantly enhance alkaline phosphatase activity (P < 0.05), and accelerate osteoblast differentiation.

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