Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (33): 6087-6090.doi: 10.3969/j.issn.1673-8225.2010.33.002

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Injection of chondrocyte transfected by bone morphogenetic protein-2 into intervertebral disc degeneration models: Contents of proteoglycan and collagen at 10 weeks

Liu Cheng-long, Jin An-min, Li Xia-lin, Du Xue-jun, Cao Yan-lin, Zhuang Ning   

  1. Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Online:2010-08-13 Published:2010-08-13
  • About author:Liu Cheng-long☆, Doctor, Lecturer, Associate chief physician, Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China Liucw878@163.com
  • Supported by:

    the Medical Research Foundation of Guangdong Province, No. B2007118*

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Abstract:

BACKGROUND: Previous studies demonstrated that bone morphogenetic protein-2 (BMP-2) transfected chondrocytes can promote nucleus pulposus cells synthesize extracellular matrix, but its effect on metabolism of degenerating intervertebral disc remains poorly understood.
OBJECTIVE: To study the feasibility of the application of BMP-2-transfected chondrocytes on intervertebral disc degeneration.
METHODS: New Zealand rabbits were prepared for intervertebral disc degeneration models. The model success was randomly divided into cell transplantation and control groups, which received injection of BMP-2-transfected chondrocyte or physiological saline, respectively. The intervertebral disc tissues were harvested at 2, 4, 6, 8 and 10 weeks after injection, and the proteoglycan and collagen contents were measured. 
RESULTS AND CONCLUSION: Compared with the control group, the proteoglycan and collagen contents of the cell transplantation group were obviously increased (P < 0.05). The findings revealed that, extracellular matrix reduce was one important indication of intervertebral disc degeneration. Transplantation of BMP-2-transfected chondrocytes can raise the content of extracellular matrix, improve biological activity of degenerative nucleus pulposus, and delay intervertebral disc degeneration.

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