Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (29): 5329-5333.doi: 10.3969/j.issn.1673-8225.2010.29.003

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Effect of microencapsulated culture on the proliferation and functional expression of hepatocytes

Huang Xiao-bo, Zhang Ying, Wang Wei, Sun Guang-wei, Zhu Jing, Xie Hong-guo, Ma Xiao-jun   

  1. Laboratory of Biomedical Material Engineering, Dalian Instiutue of Chemical Physics, Chinese Academy of Sciences, Dalian   116023, Liaoning Province, China
  • Online:2010-07-16 Published:2010-07-16
  • Contact: Ma Xiao-jun, Investigator, Laboratory of Biomedical Material Engineering, Dalian Instiutue of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning Province, China maxj@dicp.ac.cn
  • About author:Huang Xiao-bo☆, Studying for doctorate, Laboratory of Biomedical Material Engineering, Dalian Instiutue of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning Province, China huangxb@dicp.ac.cn
  • Supported by:

    National 973 Project by Ministry of Science and Technology, No. 2005CB522702*; the National Natural Science Foundation of China, No. 20736006*; Major Program of the National 863 Project, No. 2006AA02A140*

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Abstract:

BACKGROUND: The previous experiments have proved that, the configuration of hepatocarcinoma cells (HepG2) was changed during microencapsulated culture and the cellular skeleton was also rearranged.
OBJECTIVE: Based on the previous study, this study was aimed to further investigate the effect of microencapsulated culture on the proliferation and function expression of HepG2 cells.
METHODS: Human HepG2 cell lines were encapsulated in alginate-poly-L-lysine-alginate microcapsules. The configuration and microstructure of cellular spheroids, cellular proliferation and function expression were detected through microscopy and hematoxylin-eosin staining, MTT assay, Realtime RT-PCR and Elisa assay, respectively.
RESULTS AND CONCLUSION: Microencapsulated HepG2 cells aggregated into clumps with tight junctions and proliferated in three-dimensional manner. Compared with the monolayer culture, the rate of cellular proliferation in microcapsules was reduced. However, the gene expression related with liver function and albumin secretion were increased in the microencapsulated HepG2 cells. The microenvironment provided by alginate-poly-L-lysine-alginate microcapsules can contribute to in vitro construction of HepG2 cells. The alginate-poly-L-lysine-alginate microcapsules can achieve in vitro culture of hepatocytes, it is a potential pattern of three-dimensional culture, and can be used in cancer therapy, high-flux drug screening and liver tissue engineering study.

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