Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (28): 5212-5215.doi: 10.3969/j.issn.1673-8225.2010.28.019

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Establishment of diet-induced type 2 diabetes mellitus rat models

Zhang Song-yun1, Yang Chang-chun2, Hao Yong-mei1, Zhang Qing-jiu3, Jiang Hui-qing4   

  1. 1 Department of Endocrinology, Second Affiliated Hospital of Hebei University, Shijiazhuang  050000, Hebei Province, China; 2 First Department in South Building, the Armed Police General Hospital, Beijing  100039, China; 3 Department of Neurosurgery, 4Department of Gastroenterology, Second Hospital of Hebei Medical University, Shijiazhuang  050000, Hebei Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Jiang Hui-qing, Doctor, Professor, Chief physician, Department of Gastroenterology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China huiqingjiang@yahoo.com.cn
  • About author:Zhang Song-yun☆, Doctor, Associate chief physician, Associate professor, Department of Endocrinology, Second Affiliated Hospital of Hebei University, Shijiazhuang 050000, Hebei Province, China zhangsongyun2004@yahoo.com.cn
  • Supported by:

    the Medical Scientific Research Foundation of Health Department of Hebei Province, No. 07067*

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Abstract:

BACKGROUND: Fat-fed plus small dose of streptozotocin are widely used to induce rat type 2 diabetes mellitus (T2DM) models in china, while only half of fat-fed rats develop insulin resistance. Thus, it is necessary to explore ideal methods for T2DM model establishment.
OBJECTIVE: To explore a novel animal model for T2DM by selecting the diet-induced obese (DIO) rats plus streptozotocin injection.
METHODS: Sprague Dawley rats were randomly divided into the control and high fat diet groups. Four weeks later, rats of HF diet group were divided into DIO group and diet-induced obesity resistance (DR) group by body weight. Eight weeks later, rats in all groups were injected with streptozotocin intraperitoneal (30 mg/kg). Rats with fasting blood glucose >7.8mmol/L and maintained for 2 weeks were enrolled in T2DM. The levels of fasting blood glucose, total cholesterol and triglyceride, serum insulin, insulin sensitivity were determined. Diabetic successful rate was compared among groups.
RESULT AND CONCLUSION: Compared with the control and DR group, the body weight, total cholesterol, triglyceride, serum insulin and insulin resistance increased significantly in DIO group (P < 0.01). Diabetic successful rate in DIO group reached to 100%. It is a successful improvement to establish rat models of T2DM by selecting DIO rats combined with STZ injection.

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