Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (28): 5208-5211.doi: 10.3969/j.issn.1673-8225.2010.28.018

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Effect of low-energy laser irradiation at oral pharynx and Dubi acupoint (ST35) on metabolism of oxygen free radicals in rabbits with knee osteoarthritis 

Zhang Yan-jiao1, Li Shao-xin2, Guo Zhou-yi3, Huang Han-chuan3, Yang Xi-chen3   

  1. 1 Laboratory of Physiological Science, 2 Department of Physics, Guangdong Medical College, Dongguan  523808, Guangdong Province, China; 3 Laboratory of Photonic Traditional Chinese Medicine, South China Normal University, Guangzhou  510631, Guangdong Province, China
  • Online:2010-07-09 Published:2010-07-09
  • About author:Zhang Yan-jiao★, Master, Lecturer, Laboratory of Physiological Science, Guangdong Medical College, Dongguan 523808, Guangdong Province, China ajiao06@126.com
  • Supported by:

    the Science and Technology Plan of Dongguan City, No. 2007108101025*

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Abstract:

BACKGROUND: At present, laser irradiation, especially local irradiation, are used for treating knee osteoarthritis. But it has limitations in output power and signal, which easy to produce adaptation and reduce the efficacy after repeated irradiation.
OBJECTIVE: To explore the influence of the low-energy laser irradiating at acupoint or at low-energy laser multiple areas united irradiation on the metabolism of oxygen free radicals in experimental rabbits with knee osteoarthritis.
METHODS: Papain solution was injected into articular cavity of rabbits to prepare knee osteoarthritis models. Low-energy laser irradiating at Dubi acupoint (ST35) or at oral pharynx and Dubi acupoint (ST35) was applied for 15 days, 10 minutes per day. The serum levels of nitrogen monoxidum (NO), malondialdehyde (MDA) and synovium superoxide dismutase (SOD) were detected. Hematoxylin-eosin staining was performed to observe histomorphological changes of knee cartilage tissues.
RESULTS AND CONCLUSION: Compared with the control group, the contents of serum NO and MDA elevated significantly in the model group (P < 0.01 or P < 0.05), and SOD activities were decreased significantly (P < 0.05). The pathological damage mitigated after laser irradiation, NO and MDA levels were decreased, and SOD activity was increased, especially in the united irradiation group (P < 0.05). The changes of MDA levels in synovial tissue of joint was the same as serum SOD contents. Low-energy laser irradiation at oral pharynx and Dubi acupoint (ST35) can correct oxygen free radicals metabolic disorder and reduce structure damage of articular cartilage, which achieve superior results to irradiation at Dubi acupoint in treating knee osteoarthritis.

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