Abstract:

BACKGROUND: The application of injecting human skin fibroblast (HSF) cultured in vitro has provided a firenew idea for the treatment of facial wrinkles and minor depression. However, it would be more practical if the time which HSF cultured in vitro could be shortened.
OBJECTIVE: To investigate the effect of transforming growth factor-β1 (TGF-β1), insulin-like growth factor-I (IGF-Ⅰ) and their synergy on the HSF proliferation.
METHODS: HSF were cultured in vitro. The 3rd passage cells were inoculated into 96-well tissue culture plates, and treated with different concentrations of TGF-β1, IGF-Ⅰ, respectively, and the combinations of TGF-β1 and IGF-Ⅰwere established at their optimal effect concentrations. The control group was also established for comparison. The proliferation was detected at 3, 6 and 9 days by the MTT colorimetric method.
RESULTS AND CONCLUSION: The proliferating effects of two growth factors (10.0 μg/L TGF-β1, 50.0 μg/L IGF-Ⅰ) on the 3rd passage cells at 6 and 9 days were significantly better than in the control group (P < 0.05), thus, this was the optimal concentrations for growth factors. The differences between the experimental group and corresponding control groups had significance at 6 and 9 days after operation (P < 0.05). Compared with other groups, the combination groups of 10.0 μg/L TGF-β1 and 50.0 μg/L IGF-Ⅰ showed a significantly higher proliferating effect at 9 days after operation (P < 0.05). TGF-β1 and IGF-Ⅰ can promote the proliferation of the HSF, respectively, and the combinations of TGF-β1 and IGF-Ⅰat their optimal concentrations have better proliferating effects than the single growth factor.