Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (28): 5186-5190.doi: 10.3969/j.issn.1673-8225.2010.28.013

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RhoA/ROCK-I signaling pathway and expression of connective tissue growth factor in hypertrophic scar fibroblasts 

Dai Li-bing, Pan Shu, Shen Yan, Huang Yue, Liang Rong, Kang Ning, Li Ye-yang, Li Xiao-jian, Xie You-fu, Li Gang, Zhang Yan   

  1. Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou  510220, Guangdong Province, China
  • Online:2010-07-09 Published:2010-07-09
  • About author:Dai Li-bing, Technician-in-charge, Guangzhou Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, the Fourth Affiliated Hospital of Medical College, Jinan University, Guangzhou 510220, Guangdong Province, China Libingdai@126.com
  • Supported by:

    the Administration of Traditional Chinese Medicine of Guangdong Province, No. 2007401*; Foundation of Traditional and Western Medicine, No. 2007A16*; Program of Science and Technology Bureau of Guangzhou, No. 2008J1-C121*; the Natural Science Foundation of Guangdong Province, No. 9152800001000 009*; Key Program of Medical Science of Guangzhou, No. 2005-Zdi-03*, 2006-Zdi-08*,2008-Zdi-08*, 2009-Zdi-05*, 2009-Zdi-12*

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Abstract:

BACKGROUND: Our previous research has demonstrated that the expression of RhoA and ROCK-I in hypertrophic scar fibroblasts were greater than those in normal skins. These result suggested that RhoA/ROCK signaling pathway might take part in the hypertrophic scar formation. But these effects are still unclear in the hypertrophic scar formation.
OBJECTIVE: To study the effects of on expression of connective tissue growth factor (CTGF) in hypertrophic scar fibroblasts.
METHODS: Fibroblasts were obtained from human hypertrophic scars and cultured in the mediums. Transforming growth factor β1 (TGF-β1) and Y-27632 were used to intervene the cells. Fluorescent quantitative PCR assay and immunofluorescence cytochemistry were used to quantify the expression and secretion of RhocA, ROCK-I and CTGF in the hypertrophic scar fibroblasts.
RESULTS AND CONCLUSION: Upon TGF-β1 treatment, the expression and secretion of RhocA, ROCK-I and CTGF in the hypertrophic scar fibroblasts were significantly increased (P < 0.01). But these effects of TGF-β1 on ROCK-I and CTGF were inhibited in the TGF-β1+Y-27632 group. However, there were no expression changes after single Y-27632 treatment. This indicated that TGF-β1 can regulate the expression of CTGF via RhoA/ROCK-I signaling pathway, namely, RhoA/ROCK-I signaling pathway participate the expression regulation of CTGF in hypertrophic scar fibroblasts and block the downstream pathway, which may be one of the molecular mechanism of TGF-β1 in effecting hypertrophic scar formation.

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