Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (28): 5181-5185.doi: 10.3969/j.issn.1673-8225.2010.28.012

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Expression of vascular endothelial growth factor in fracture healing rats combined with brain injury  

Wu Cheng-long1, Guan Jian2, Song Yong-zhou1, Feng Guo-jun1, Ma Wei1   

  1. 1 The Second Hospital of Hebei Medical University, Shijiazhuang  050000, Hebei Province, China; 2 The Third Hospital of Shijiazhuang, Shijiazhuang  050000, Hebei Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Ma Wei, Professor, Master’s supervisor, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China mw0632@sohu.com
  • About author:Wu Cheng-long★, Studying for master’s degree, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China chlongwu1015@sohu.com

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Abstract:

BACKGROUND: Many clinical cases demonstrated that the callus number was obvious increased, and the bone healing was faster in fracture patients combined with brain injury. However, there is not a prospective control study underlying the effects of vascular endothelial growth factor (VEGF), and the mechanism is unclearly.
OBJECTIVE: To explore the changes of VEGF expression in SD rats fracture healing combined with brain injury and fracture only.
METHODS: SD rats were randomly divided into 3 groups: normal control, simple fracture and fracture combined with brain injury groups. Rat diffuse midrange brain injured model was prepared by using modified Marmarou installation method. In addition, rat fracture model was prepared as follow: Knee joint of left lower extremity was depilated and sterilized, Condyles of tibia was perforated, shin bone was fracted transversally in above 1/3 of shin bone. The reparative process and effect of fracture were observed by X-ray films; and VEGF expression in the Osteotylus samples were detected by RT-PCR and immunohistochemistry staining.
RESULTS AND CONCLUSION: There were no obviously differences between the simple fracture and fracture combined with brain injury groups at days 3, 7 and 14 after fracture. Compared to the simple fracture group, the fracture lines of the brain injury combined fracture group have become blurred, and formed a great quantity osteotylus surrounding the fracture parts at day 24, and the fracture lines disappeared after 42 days, the fracture has basically healed. In the simple fracture group, the expression of VEGF appeared at day 7, gradually increased, reached a peak at week 3, but disappeared after 42 days. In the brain injury combined fracture group, the expression of VEGF appeared at day 3, and reached a peak 2-3 weeks later. Its peak expression was early and lasted for a longer time. It demonstrated that the bone healing was faster in fracture rat combined with brain injury, namely, brain injury has promoted effect on fracture healing, which may relevant to longer persistence time and ahead of schedule of VEGF expression.

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