Abstract:

BACKGROUND: Previous studies showed that electric fields affect cell shape, cell motility and morphology, including endothelial cell proliferation and directional migration. This effect plays an important role in the angiogenesis and vascular injury repair system.
OBJECTIVE: To explore the effect of external direct current (DC) fields on the proliferation and migration of human umbilical vein endothelial cells HY926.
METHODS: MTT assay was performed to detect in the proliferation of human umbilical vein endothelial cells HY926 under the voltage of 3 V, 5 V, 8 V, 10 V roles for 12 hours. The movement trajectory of the human umbilical vein endothelial cells under the voltage of 5 V for 10 hours were tracked and analyzed statistically, including the average cumulative distance and the average rate of cell migration, the average cell size and direction of the ultimate displacement (indicated using the sine value), and the average sine value of all single cell in an hour.
RESULTS AND CONCLUSION: After treatment with the voltage of 3 V, 5 V, 8 V, 10 V for 12 hours, the cells growth were inhibited remarkably. However, having been applied voltage of 5 V, the electric field strength of 163.46 mV / cm, the proliferation of endothelial cells performed the best. In the physical electric field, the average migration rate of endothelial cells showed a downward trend with the time increasing. After applied voltage of 5 V for 10 hours, all most of the endothelial cells obviously migrated towards the cathode continuously. It suggested that the applied DC electric field significantly inhibited the proliferation of the human umbilical vein endothelial cells, and induced cell migration directed to the cathode.