Abstract:

BACKGROUND: The pathogenesis of diabetic cardiomyopathy is unclear. Neuregulin-1 (NRG-1) can promote revascularization. Whether the incidence of diabetes cardiomyopathy is relevant to the down expression of NRG-1 of cardiac microvascular endothelial cells needs further research.
OBJECTIVE: To observe the effect of high-glucose on the expression of the NRG-1 of cardiac microvascular endothelial cells.
METHODS: The second generation of cardiac microvascular endothelial cells were selected and divided into three groups. Control group: cells were cultured in normal medium; high-glucose group: glucose (10 mmol/L) was added into medium;  glucose + insulin group: glucose (10 mmol/L) and insulin (10-5 U/L) were added into medium. Cardiac microvascular endothelial cells were collected after 24 hours, RT-PCR and Western blot were used to detect the expression of NRG-1 mRNA and protein. 
RESULTS AND CONCLUSION: Compared with the control group, the expression of the NRG-1 mRNA and NRG-1 protein in the high-glucose group were decreased significantly (P < 0.05). Compared with high-glucose group, the expression of the NRG-1 mRNA and NRG-1 protein in the glucose + insulin group were increased significantly (P < 0.05). Compared with the control group, the difference was not significant (P > 0.05). High glucose can inhibit the expression of NRG-1 mRNA and NRG-1 protein of cardiac microvascular endothelial cells, which can be antagonized by insulin.