Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (28): 5151-5154.doi: 10.3969/j.issn.1673-8225.2010.28.005

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Rabbit knee osteoarthritis model induced by anterior cruciate ligament transaction: Protective effects of alendronate combined with glucosamine hydrochloride on articular cartilage and subchondral bone  

Pi Jun-jie1, Lü Zhi-wei2, Yan Zhi-rong3, Lu Kai-lin4, Cai Hai-feng5   

  1. 1 Department of Surgery, 3 Department of Pharmacy, Branch of Kailuan Hospital, Tangshan  063000, Hebei Province, China; 2 Department of Anatomy, North China Coal Medical University, Tangshan  063000, Hebei Province, China; 4 Department of Surgery, Kailuan Hospital, Tangshan  063000, Hebei Province, China; 5 Department of Oncology, Tangshan People’s Hospital, Tangshan  063000, Hebei Province, China
  • Online:2010-07-09 Published:2010-07-09
  • About author:Pi Jun-jie, Attending physician, Department of Surgery, Branch of Kailuan Hospital, Tangshan 063000, Hebei Province, China pjj123654@sohu.com

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Abstract:

BACKGROUND: Glucosamine hydrochloride is considered available agent for osteoarthritis, while alendronate, an inhibitor of bone resorption, is reported to be potential for the treatment of osteoarthritis.
OBJECTIVE: To study the curative effects and related mechanisms of alendronate combined with glucosamine hydrochloride on the osteoarthritic rabbit knee induced by anterior cruciate ligament transaction.
METHODS: New Zealand rabbits, aged 3 months, were divided randomly into sham surgery, model, glucosamine hydrochloride, and glucosamine hydrochloride + alendronate groups. All rabbits were prepared for right knee osteoarthritis model induced by anterior cruciate ligament transaction except that in the sham surgery group. Group glucosamine hydrochloride received a daily administration of glucosamine hydrochloride (150 mg/kg), group glucosamine hydrochloride + alendronate received glucosamine hydrochloride plus a subcutaneous injection of alendronate (10 μg/kg per day); in contrast, animal in the model group received normal saline with the same dose. All rabbits were sacrificed 8 weeks later. Samples of cartilage harvested from right knees were observed for the macro-pathologic changes after the first and the right tibias were removed and biomechanical test was performed to measure the maximal loading and compression modulus. Femurs were prepared for the paraffin section, with the subsequent staining of hematoxylin-eosin staining and graded by Mankin scale.
RESULTS AND CONCLUSION: Compared to the model group, rabbits in the glucosamine hydrochloride group and glucosamine hydrochloride + alendronate group showed milder osteoarthritic changes, with significantly lower Mankin scores (P < 0.05). Bone mineral density of distal femur in the glucosamine hydrochloride + alendronate group was significantly higher than those of other groups (P < 0.05). Both the maximal loading and compression modulus in the glucosamine hydrochloride group was significantly lower than those of glucosamine hydrochloride + alendronate group. Alendronate combined with glucosamine hydrochloride had a better effect on anterior cruciate ligament transaction-induced rabbit osteoarthritis by inhibiting the development of osteoarthritis from directly protecting cartilage and modulating subchondral bone metabolism.

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