Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (28): 5141-5145.doi: 10.3969/j.issn.1673-8225.2010.28.003

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Effect of compound stanozolol on different skeletal sites of rats induced by D-galactose

Zhang Xin-le, Wu Tie, Cui Liao, He De-mou, Li Bin, Zhang Yi-jia, Wu Guang-liang, Dai Juan-xiu   

  1. Department of Pharmacology, Guangdong Medical College, Zhanjiang  524023, Guangdong Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Wu Tie, Professor, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
  • About author: Zhang Xin-le★, Master, Laboratory technician, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China xinlezhang@tom.com
  • Supported by:

     Science and Technology Planning Project of Zhanjiang City, No. 2008C04016*

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Abstract:

BACKGROUND: Stanozolol can increase bone mineral density in osteoporosis patients, which promotes bone formation and inhibiting bone resorption, however, its effectiveness in senile osteoporosis, as well as its effects on the different parts of the skeleton, remains unclearly.
OBJECTIVE: To study the effect of compound stanozolol on different skeletal sites of rat D-galactose-induced osteoporosis model through bone histomorphometry observation.
METHODS: Sprague Dawley rats were divided into the normal control, D-galactose and compound stanozolol groups according to random number table. All rats were prepared osteoporosis models by subcutaneous injecting D-galactose exception of the normal control group. In the compound stanozolol group, rats were gastric irrigated with stanozolol 0.54 mg/(kg•d)+Piracetam
432 mg/(kg•d) for 14 successive weeks. Solvent control was performed between normal control and D-galactose group. Bone histomorphometric parameter of the proximal tibial metaphysis (PTM), and tibial shaft was calculated. The biomechanic properties of right femur were analyzed by three-point bending test.
RESULTS AND CONCLUSION: Bone histomorphometric analysis showed that compound stanozolol could prevent micro-structural damage of PTM caused by D-galactose, inhibit bone resorption and facilitate proximal tibial bone formation. However, compound stanozolol had no obviously effect on cortical bone mass loss of tibial shaft by D-galactose.

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