Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (15): 2705-2808.doi: 10.3969/j.issn.1673-8225.2010.15.011

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Effects of erythropoietin on biological characteristics of rat endothelial progenitor cells  

Wang Dong1, Wang Liang1, Liu Qing-guo2, Wang Xiao-nan1, Wei Hui-jie1, Zhang Jian-ning1   

  1. 1 Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin  300052, China; 2 Department of Neurosurgery, Affiliated Heping Hospital of Changzhi Medical College, Changzhi  047100, Shanxi Province, China 
  • Online:2010-04-09 Published:2010-04-09
  • Contact: Zhang Jian-ning, Professor, Doctoral supervisor, Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin 300052, China jianningzhang@hotmail.com
  • About author:Wang Dong★, Studying for master’s degree, Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin 300052, China 54454241@qq.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30772229*; Doctoral Program Foundation of Institutions of Higher Education of China, No. 20070062008*

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Abstract:

BACKGROUND: Endothelial progenitor cells (EPCs) is the progenitor cells of endothelial cells, which participated in angiogenesis. Some researches demonstrate that recombinant human erythropoietin (rhEPO) can mobilize EPCs from bone marrow, and have a beneficial effect on recovery of injury tissue.
OBJECTIVE: To investigate the effect of rhEPO on the function of rats EPCs.
METHODS: Bone marrow was obtained from both femurs and tibials of Wistar rats, and mononuclear cells were isolated by density gradient centrifugation, followed by cultured and treated with several does of rhEPO (0, 4, 8 U/mL). At 7 days after culture, EPCs were identified by FITC-UEA-1 and Dil-Ac-LDL co-staining. Meanwhile, EPCs were marked by CD133 and CD34, identified by flow cytometry; additionally, the functions of EPCs were evaluated using adhesion test, migration assay and proliferation experiment.
RESULTS AND CONCLUSION: At 7 days after culture, the cell morphology changed completely, and formed a tube-like structure, which presented typical cobblestone appearance after cell conjugation. The fluorescence staining showed that the EPCs were positive to both FITC-UEA-1 and Dil-Ac-LDL co-staining. The CD133 and CD34 cells were combined to EPCs by flow cytometry. Finally, the adhesion test, migration assay and proliferation experiment demonstrated that rhEPO could improve adhesion, migration and proliferative capacities in a dose-dependent manner. The results demonstrated that rhEPO can significantly enhance the functions of EPCs in dose-dependent manner at 0-8 U/mL concentration.

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