Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (11): 1987-1991.doi: 10.3969/j.issn.1673-8225.2010.11.022

Previous Articles     Next Articles

Influence of beta nerve growth factor in vibrissa follicle bulge region on the burn wound healing of neonatal rats

Zeng Jun1, Chen Deng-bang1, Dai Lü-xia1, Lin Sen2   

  1. 1Clinic Experiment Teaching Center of Chengdu Medical College, Chengdu  610083, Sichuan Province, China;
    2Department of Human Anatomy, Histology and Embryology, Chengdu Medical College, Chengdu  610083, Sichuan Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Lin Sen, Master, Lecturer, Department of Human Anatomy, Histology and Embryology, Chengdu Medical College, Chengdu 610083, Sichuan Province, China lsroger@163.com
  • About author:Zeng Jun, Assistant laboratory technician, Clinic Experiment Teaching Center of Chengdu Medical College, Chengdu 610083, Sichuan Province, China janezj6@hotmail.com
  • Supported by:

    the Youth Fund Project of Education Department of Sichuan Province, No. 07ZB016*

PDF

734

Abstract:

BACKGROUND: The surroundings stimulation, such as wound and chemical injury, will result in changes of hair follicle stem cells (HFSC). Up to date, few articles indicate the effect of burn on HFSCs. 
OBJECTIVE: To investigate the changes of β-nerve growth factor (β-NGF) level in vibrissa follicle bulge region and its effect on the healing of neonatal rats.
METHODS: A total of 30 neonatal rats were selected to prepare burn models by giving 90 ℃ boiled tap water for 3 seconds, and vibrissa follicle bulge was separated at 12, 24, 36, 48 and 60 hours postoperatively. The remained 6 neonatal rats were served as controls. Total protein concentration was measured by Brandford method. The expression of β-NGF was detected by ELISA. Meanwhile, the tissues in each time points were collected for frozen section and immunohistochemistry.
RESULTS AND CONCLUSION: Among the tested total protein, the expression of the β-NGF increased after 36 hours, reached a peak at 60 hours after burn. The identical changes were found in tissue slices. The image analysis demonstrated that the β-NGF protein detection was coincident with β-NGF expression in tissues. During 12 hours to 60 hours after burn, the expression of the β-NGF in the bulge area peaked at 60 hours; simultaneously, the repairing effect of healing also reached a peak. Bulge area is a nest of HFSC, the enhanced expression of β-NGF may have some positive significance to the differentiation of the HFSC and tissue repair.

CLC Number: