Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (11): 1992-1994.doi: 10.3969/j.issn.1673-8225.2010.11.023

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May HOXD13-zinc finger protein 548 complex develop in foot tissue during human embryonic development?

Wang Li-li1, Xin Na2, Sun Kai-lai2   

  1. 1Experimental Research Center, Shengjing Hospital Affiliated to China Medical University, Shenyang  110004, Liaoning Province, China;
    2Department of Medical Genetics, Basic Medical College, China Medical University, Shenyang   110001, Liaoning Province, China
  • Online:2010-03-12 Published:2010-03-12
  • About author:Wang Li-li, Doctor, Experimental Research Center, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China wll_1977_21@163.com
  • Supported by:

    the Sub-project of National Basic Research and Development Program of China, No. 2001CB510301*

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Abstract:

BACKGROUND: The HOX gene family is a highly conserved transcription factor family, which affects the formation of basic axis and secondary axis during embryonic development, at the same time, it plays a pivotal role in the development of the central nervous system, axial skeleton, stomach intestine, urogenital system and external genitalia.
OBJECTIVE: To investigate the interactional protein of HOXD13 in human embryo foot development at 14 weeks pregnancy.
METHODS: Fetal foot tissues were harvested and protein extraction was performed using protein extraction kit, 6 μg HOXD13 antibody was added for immunoprecipitation experiment, which aims to obtain HOXD13 interactional proteins, while the non-specific IgG served as a negative control. Subsequent to Bradford staining, the sediment protein bands were cut for mass spectrometry.
RESULTS AND CONCLUSION: We obtained an interactional protein of HOXD13 in the human embryo foot at 14 weeks pregnancy, at the relative molecular mass of approximately 60 000. The protein was identified as zinc finger protein 548 using mass spectrum. HOXD13 may play a very important role in transcriptional control by HOXD13-zinc finger protein 548 protein complex in the human embryo foot at 14 weeks pregnancy.

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