Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (11): 1995-1999.doi: 10.3969/j.issn.1673-8225.2010.11.024

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Effects of heparinase Ⅰ on syndcan-1 and extracelluar signal regulated kinase expression in human umbilical vein endothelial cells following hypoxia/reoxygenation

Luo Hai-yan, Hu Chang-lin   

  1. Department of Neurology, Second Hospital of Chongqing University of Medical Sciences, Chongqing   400010, China
  • Online:2010-03-12 Published:2010-03-12
  • About author:Luo Hai-yan, Doctor, Associate professor, Associate chief physician, Master’s supervisor, Department of Neurology, Second Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China luohy1965@163.com
  • Supported by:

    the Medical Scientific Education Program of Chongqing Health Bureau, No. 2005-31*;
    the Science and Technology of Chongqing Medical University, No. XB-0518*

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Abstract:

BACKGROUND: Heparinase can induce syndecan-1 shedding from tumor cells, and macrophage motion may correlate with biosynthesis regulation of heparan sulfate proteoglycan.
OBJECTIVE: To investigate the effects of heparinaseⅠon syndecan-1 and extracelluar signal regulated kinase 2 (ERK2) in human umbilical vein endothelial cells (HUVECS) with hypoxia/reoxygenation injury.
METHODS: heparinaseⅠ-precultured HUVECS were treated with hypoxia/reoxygenation. Immunohistochemistry staining, RT-PCR and western blot were applied to detect HUVECS syndecan-1 and ERK2 expression.
RESULTS AND CONCLUSION: Expression of syndecan-1 and ERK2 was increased in HUVECS following hypoxia/reoxygenation treatment. HeparinaseⅠ significantly upregulated the expression of syndecan-1 and ERK2 in HUVECS with hypoxia/reoxygenation treatment. Syndecan-1 and ERK2 expression was positively related. Results show that syndecan-1 and ERK2 participate the pathophysiology of HUVECs hypoxia/reoxygenation injury. HeparinaseⅠ influences ERK2 expression by regulating syndecan-1.

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