Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (11): 1927-1930.doi: 10.3969/j.issn.1673-8225.2010.11.007

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Expressions of Smad3 and transforming growth factor beta 1 in keloid, hypertrophic scars and normal skins: A 48: 40: 40 sample pathological detection

Pang Jiu-ling1, Ma Zheng2, Liu Jun1, Liu Ai-dong2   

  1. 1Department of Burn and Plastic Surgery, Workers’ Hospital of Tangshan, Hebei Medical University, Tangshan  063000, Hebei Province, China;
    2Medical Campus of Tangshan Vocational and Technical College, Tangshan  063000, Hebei Province, China
  • Online:2010-03-12 Published:2010-03-12
  • About author:Pang Jiu-ling, Chief nurse, Department of Burn and Plastic Surgery, Workers’ Hospital of Tangshan, Hebei Medical University, Tangshan 063000, Hebei Province, China lad303@163.com

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Abstract:

OBJECTIVE: Study regarding Smad3/transforming growth factor-β1 (TGF-β1) signal transduction in pathological scars mainly focus on in vitro cultured fibroblasts, however, the correlation study was rare on keloid. The aim of this study is to detect the expressions of Smad3 and TGF-β1, and investigate their relationship in pathogenesis and development of pathological scars.

METHODS: Experimental samples were obtained from the patients, who underwent burn and plastic surgery at the Department of Burn and Plastic Surgery, Workers’ Hospital of Tangshan, Hebei Medical University, from June 2004 to June 2008, including 48 patients with Keloid, aged 16-52 years, and 40 patients with hypertrophic scars aged 18-56 years. Normal skins from additional 40 cases were served as controls. The expressions of Smad3 and TGF-β1 protein in keloid, hypertrophic scars and normal skin were examined by flow cytometry.

RESULTS: The expression of Smad3 and TGF-β1 were obviously greater in the experimental group than that of the control group (P < 0.05), but the difference between keloid and hypertrophic scars had no significance (P > 0.05). There was a positive correlation between Smad3 and TGF-β1 in keloid and hypertrophic scars (r=0.489 2, P=0.000 4; r=0.471 0, P=0.002 2). No notable correlation was found between Smad3 and TGF-β1 in normal skins (P=0.471 4).

CONCLUSION: The expressions of Smad3 and TGF-β1 are up-regulated, and the synergism of Smad3 and TGF-β1 may promote the development in pathological scars.

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