Abstract:

BACKGROUND: In recent years, pentoxifylline has been found to have a wide range of anti-fibrosis capacity. However, there are few studies explore the suppress effect of pentoxifylline on fibroblasts in human keloid, and the maximum inhibitory concentration remains poorly understood.
OBJECTIVE: To study the effect of pentoxifylline on proliferation activity of human keloid fibroblasts and to select the maximum inhibitory concentration.
METHODS: Human keloid fibroblasts were used as original cells, passaged till the 5th to the 8th generations, and then divided into the experimental and control groups. Pentoxifylline with concentrations of 0.1, 0.25, 0.5, 1.0, 2.0 and 3.0 g/L were added to the experimental group. The effects of different concentrations of pentoxifylline on proliferation of keloid fibroblasts were detected by MTT chromometry.
RESULT AND CONCLUSION: Compared with the control group, the inhibitory effect of pentoxifylline on the proliferation of keloid fibroblasts was more evident in the experimental group (P < 0.05). The inhibition rates of pentoxifylline on proliferation of keloid fibroblasts showed apparently time- and dose-effect relationships within the concentration of 0.1- 2.0 g/L, which presented a greatest level at 96 hours after culture. The maximum inhibitory rate was 53.37%, and the concentration was 2.0 g/L in the experimental group. Consequently, pentoxifylline plays a notable inhibitory role in the proliferation of human keloid fibroblasts with concentration of 2.0 g/L at 96 hours after culture.