Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (2): 379-384.doi: 10.12307/2025.271

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Advantages of modified ligation method for spinal cord injury modeling 

Li Daohui1, Xu Xiaoshuang2, Li Zhengtao1, Tian Xinpeng3, Bi Hangchuan1, Liu Yuan1, Dai Yongwen1, Chen Lingqiang1   

  1. 1Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China; 2School of Public Health, Kunming Medical University, Kunming 650500, Yunnan Province, China; 3Xichang People’s Hospital, Liangshan Yi Autonomous Prefecture 615000, Sichuan Province, China
  • Received:2023-12-08 Accepted:2024-02-08 Online:2025-01-18 Published:2024-05-25
  • Contact: Chen Lingqiang, Professor, Doctoral supervisor, Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Co-corresponding author: Dai Yongwen, Master candidate, Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Li Daohui, Master candidate, Department of Orthopaedics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Xu Xiaoshuang, Master candidate, School of Public Health, Kunming Medical University, Kunming 650500, Yunnan Province, China Li Daohui and Xu Xiaoshuang contributed equally to this work.
  • Supported by:
    National Natural Science Foundation of China, Nos. 81660215 and 81860093 (to CLQ); Science and Technology Programs of Yunnan Provincial Science and Technology Department, Nos. 202101AT070226 and 202101AY070001-31 (to CLQ)

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Abstract: BACKGROUND: Currently, different methods of model establishment have been derived from different injury modes of spinal cord injury. Traditional physical injury modeling methods have their own advantages and disadvantages, and there is a lack of more effective and stable animal models of spinal cord injury.
OBJECTIVE: To establish a reproducible, controllable, trauma-free, low-mortality, more stable, widely applicable, and short-term postoperative care rat model of spinal cord injury.
METHODS: Forty Sprague-Dawley rats with similar body mass and ages were randomly divided into a control group and an improved group, with 20 rats in each group. Animal models of spinal cord injury in the control group were constructed using a clip model method, while the improved group used a modified ligation method based on the compression method to make the spinal cord injury models using suture ligation based on fenestration. Postoperative comparisons were made between the two groups, assessing urination behavior, hematuria, pyuria (infection rate), mortality, scoliosis rate and Basso-Beattie-Bresnahan locomotor rating scale scores at 1, 3, 5, and 7 days after modeling.
RESULTS AND CONCLUSION: Compared with the conventional modeling method, the modified ligation method based on the compression method resulted in faster recovery of urination behavior, lower hematuria rate, lower infection rate, lower mortality rate, lower scoliosis rate, and more concentrated and stable Basso-Beattie-Bresnahan scores (all below 2 points within 1 week). This proves that the modified ligation method based on compression is more suitable for the establishment of spinal cord injury models in rats.

Key words: improvement, spinal cord injury model, clip model method, ligation method, urination reflex, urination behavior, scoliosis, BBB score

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