Comparison of biological characteristics of adipose-derived stem cells in young and old mice

doi:10.12307/2025.076

Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (19): 4063-4068.doi: 10.12307/2025.076

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Comparison of biological characteristics of adipose-derived stem cells in young and old mice

Lin Meiyu1, 2, 3, Yao Xiang1, 2, 3, Gao Jing1, 2, 3, Zhao Xilong1, 2, 3, Pan Xinghua1, 2, 3, Ruan Guangping1, 2, 3

1Basic Medical Laboratory of 920 Hospital of the Joint Logistics Support Force of Chinese PLA, Kunming 650032, Yunnan Province, China; 2Integrated Engineering Research Center of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; 3Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China

Received:2024-03-15 Accepted:2024-05-07 Online:2025-07-08 Published:2024-09-13
Contact: Ruan Guangping, MD, Associate chief physician, Basic Medical Laboratory of 920 Hospital of the Joint Logistics Support Force of Chinese PLA, Kunming 650032, Yunnan Province, China; Integrated Engineering Research Center of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China
About author:Lin Meiyu, Master candidate, Basic Medical Laboratory of 920 Hospital of the Joint Logistics Support Force of Chinese PLA, Kunming 650032, Yunnan Province, China; Integrated Engineering Research Center of Cell Biological Medicine of State and Regions, Kunming 650032, Yunnan Province, China; Transfer Medicine Key Laboratory of Cell Therapy Technology of Yunan Province, Kunming 650032, Yunnan Province, China
Supported by:
Major Science and Technology Project of Yunnan Science and Technology Plan Project, No. 2018ZF007 (to PXH); Key Project of Yunnan Province, No. 202101AS070039 (to RGP); In-Hospital Technology Plan of 920 Hospital of the Joint Logistics Support Force of Chinese PLA, No. 2020YGD12 (to RGP)

Abstract

Abstract: BACKGROUND: Adipose-derived stem cells have anti-aging effects, but whether adipose-derived stem cells from donors of different ages are different needs further study.
OBJECTIVE: To compare the biological properties of adipose-derived stem cells in old and young mice.
METHODS: Adipose-derived stem cells were extracted from adipose tissue of C57BL mice aged 8 and 14 weeks, respectively. The differences of cell cycle, apoptosis, and proliferation of adipose-derived stem cells in old and young mice were compared. The expression levels of aging-related P21 and P27 genes and proteins of adipose-derived stem cells in old and young mice were detected by quantitative PCR and western blot assay.
RESULTS AND CONCLUSION: Compared with old mouse adipose-derived stem cells, young mouse adipose-derived stem cells were more active, more regular in morphology, less apoptosis, faster proliferation, and lower in expression of age-related P21 and P27 genes and proteins. It has been proven that adipose-derived stem cells from young mice have better anti-aging effects.

Key words: adipose-derived stem cell, adipose tissue, young, old age, mouse, anti-aging

CLC Number:

Lin Meiyu, Yao Xiang, Gao Jing, Zhao Xilong, Pan Xinghua, Ruan Guangping. Comparison of biological characteristics of adipose-derived stem cells in young and old mice[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(19): 4063-4068.

Figures/Tables 2

References

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Comparison of biological characteristics of adipose-derived stem cells in young and old mice

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