Serum proteomics based on isobaric tags for relative and absolute quantitation technology in patients with nontraumatic osteonecrosis of the femoral head

doi:10.12307/2023.662

Abstract

Abstract: BACKGROUND: Nontraumatic osteonecrosis of the femoral head is a group of diseases characterized by progressive worsening of hip pain and dysfunction, which seriously endangers human health. Its pathogenesis, early diagnosis and treatment remain a major challenge for clinical orthopedic surgeons.
OBJECTIVE: Using isobaric tags for relative and absolute quantitation technology, proteomics was used to screen key proteins that may be associated with nontraumatic osteonecrosis of the femoral head. Analysis of the expression characteristics and potential biological functions of these proteins laid the foundation for further research on the etiology and molecular mechanisms of nontraumatic osteonecrosis of the femoral head.
METHODS: Serum proteins were extracted from nontraumatic osteonecrosis of the femoral head patients and healthy humans, and then labeled using isobaric tags for relative and absolute quantitation technology. Liquid chromatography combined with tandem mass spectrometry was performed to separate and analyze the peptides. Differentially expressed proteins were obtained through identifying and quantifying the proteins using Proteome Discoverer 2.1 software and analyzed via GO annotation, KEGG pathway annotation, and functional enrichment clustering analysis.
RESULTS AND CONCLUSION: (1) A total of 137 differentially expressed proteins were obtained from 1 006 proteins identified by mass spectrometry, of which the expression of 54 and 83 proteins was up-regulated and down-regulated, respectively. (2) The differentially expressed proteins associated with the development of nontraumatic osteonecrosis of the femoral head included apolipoprotein B, apolipoprotein A2, apolipoprotein E, complement C4 and C7, etc. (3) The analysis results of KEGG signaling pathway enrichment showed that the major signaling pathways of key targets comprised p53 signaling pathway, transcriptional growth factor β signaling pathway, and dilated cardiomyopathy signaling pathway. It is concluded that apolipoproteins were closely related to nontraumatic osteonecrosis of the femoral head. This study provides insight into the molecular mechanisms underlying nontraumatic osteonecrosis of the femoral head.

Key words: nontraumatic osteonecrosis of the femoral head, isobaric tags for relative and absolute quantitation, mass spectrometry, differentially expressed protein

CLC Number:

Zhang Enjing, Cai Jinkui, Xu Haijia, Li Tianhang, Li Zhanghua. Serum proteomics based on isobaric tags for relative and absolute quantitation technology in patients with nontraumatic osteonecrosis of the femoral head[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(29): 4664-4669.

Figures/Tables 6

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