Differential protein expression after acute spinal cord injury

doi:10.3969/j.issn.2095-4344.2013.41.010

Abstract

Abstract:

BACKGROUND: In mass spectrometry analysis, the same protein in different samples labeled with isobaric tags for relative and absolute quantitation presents the same mass-to-charge ratio, while in the tandem mass spectrometry analysis, the ion signal shows different mass-to-charge ratio (114-121). Thus the quantitative information of the related proteins can be obtained.
OBJECTIVE: To establish the protein spectrum of spinal cord tissue differences proteins after acute spinal cord injury, and to explore the spinal differential protein expression on the molecular level using isobaric tags for relative and absolute quantitation combined with LC-MS/MS mass spectrometry technique.
METHODS: Eight Sprague Dawley rats were selected to establish the acute spinal cord injury models using Allen’s method. The rats were randomly divided into 0 hour spinal cord injury group and 8 hours spinal cord injury group, four rats in each group. The spinal cord tissues were collected after injury, and the spinal cord tissue differences proteins were analyzed with isobaric tags for relative and absolute quantitation technique after acute spinal cord injury.

RESULTS AND CONCLUSION: Totally 220 differentially expressed proteins were identified in this research, the number of up-regulation proteins was 116 and the number of down-regulation proteins was 104. There were 12 differential proteins related to neural regeneration, and among the 12 proteins, there were seven up-regulation proteins and five down-regulation proteins. The various identified differential proteins and significantly expressed nerve growth factors in this experiment can be used as the biomarkers of acute spinal cord injury or used as the strong evidence for the clinical management and monitoring of the injury process and target therapy of acute spinal cord injury, as well as the effect evaluation.

Key words: spinal cord injuries, nerve regeneration, proteomics, mass spectrometry

CLC Number:

R318

Xu Lei-lei, Zhou Yi-jun, Song Xing-hua, Ding Li-wen, Chen Jiang-tao, Wang Chong, Gan Yu-ling, Zhu Xiao-meng. Differential protein expression after acute spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(41): 7248-7254.

Figures/Tables 3

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