Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (30): 5543-5546.doi: 10.3969/j.issn.1673-8225.2011.30.009

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Proliferation of mouse osteoblasts and expression of receptor activator of nuclear factor kappa B ligand and osteoprotegerin induced by cobalt and chromium ions

Li Jun-ning, Yuan Xiao-jun, Xu Wen-hua, Fu Yun-gen, Cao Sheng-sheng   

  1. Department of Orthopedics, the People’s Hospital of Yichun City, Yichun  336000, Jiangxi Province, China
  • Received:2011-03-27 Revised:2011-05-11 Online:2011-07-23 Published:2011-07-23
  • Contact: Yuan Xiao-jun, Master, Department of Orthopedics, the People’s Hospital of Yichun City, Yichun 336000, Jiangxi Province, China YXJ7491@163.com
  • About author:Li Jun-ning★, Master, Associate professor, Department of Orthopedics, the People’s Hospital of Yichun City, Yichun 336000, Jiangxi Province, China junning1964@163.com

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Abstract:

BACKGROUND: Metal-metal prosthesis can appear with corrosion or wear, and release nickel, cobalt, chromium, titanium, and some inflammatory factors locally.
OBJECTIVE: To investigate the effects of cobalt (Co2+) and chromium (Cr3+) ions on the proliferation of mouse osteoblasts and expression of receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG).
METHODS: Osteoblasts cultured in vitro were divided into two groups: control group with normal saline and ion group with cobalt and chromium ions.
RESULTS AND CONCLUSION: The number of cells in the control group was increased remarkably at 1-6 days after intervention, while no changes occurred in the ion group. RT-PCR results showed that the expression of RANKL and OPG was increased as compared with control group at 24 and 48 hours after coculture, especially RANKL expression (P < 0.05). The ratio of RANKL/OPG mRNA was also improved. Co2+ and Cr3+ ions have the remarkable inhibitory action on osteoblast proliferation and can stimulate the expression of RANKL and OPG mRNA from osteoblasts.

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