Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (34): 6374-6376.doi: 10.3969/j.issn.1673-8225.2010.34.025

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Active targeting behaviors of biotinylated poly(ethylene oxide)/poly(lactic acid) nanoparticles in vitro

Gong Yan-chun, Xiong Xiang-yuan, Li Zi-ling, Li Yu-ping, Guo Liang   

  1. School of Life Science, Jiangxi Science & Technology Normal University, Nanchang   330013, Jiangxi Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Xiong Xiang-yuan, Doctor, Professor, School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi Province, China xyxiong@gmail.com
  • About author:Gong Yan-chun★, Master, Teaching assistant, School of Life Science, Jiangxi Science & Technology Normal University, Nanchang 330013, Jiangxi Province, China spoilming@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 50763003*; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, No.[2007]1108*; the Preferred Scientific Research Foundation of Studying Abroad by the State Human Resource Ministry, No. [2006]164*; Natural (Young) Science Foundation of Jiangxi Province, No. 2007GQC1094*; Natural Science Foundation of Jiangxi Science & Technology Normal University, No. KY2008ZY09*

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Abstract:

BACKGROUND: Recently, the majority of polymer drug carriers have no targeting, thus are limited in applications. A few foreign research groups reported in vitro targeting behaviors of biotinylated poly(ethylene oxide)-poly(lactic acid) (Biotin-PEO-PLA) nanoparticles, however, the domestic study is absent.
OBJECTIVE: To verify the feasibility of Biotin-PEO-PLA nanoparticles as targeted drug carriers.
METHODS: Paclitaxel-loaded Biotin-PEO-PLA nanoparticles were prepared by dialysis method and characterized. The in vitro release behaviors of paclitaxel-loaded Biotin-PEO-PLA nanoparticles were studied by high performance liquid chromatogram technique. The active targeting properties of paclitaxel-loaded Biotin-PEO-PLA nanoparticles in vitro over OVCAR-3 (CA-125 antigen positive) and SKOV-3 cells (CA-125 antigen negative) were also investigated through a three-step biotin-avidin interaction by MTT tests.
RESULTS AND CONCLUSION: Paclitaxel loaded in Biotin-PEO-PLA nanoparticles shows an initial rapid release followed by a slow release period. Compared with SKOV-3 cells (CA-125 antigen negative), the survival rate of OVCAR-3 through a three-step treatment was remarkably decreased. The cytotoxicity results implied that paclitaxel-loaded Biotin-PEO-PLA nanoparticles were delivered more effectively to OVCAR-3 cells (CA-125 antigen positive) under the specific interaction between the biotin groups on the surface of Biotin-PEO-PLA nanoparticles and the avidin/biotinylated MAb X306/CA-125 antigen complexes on the surface of OVCAR-3 cells.

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