Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 5918-5922.doi: 10.3969/j.issn.1673-8225.2010.32.006

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Cocultivation of human cord blood mononuclear cells and gastric cancer cells: Screening of optimal effector/target ratio

Wu Shi-kai 1,2,3, Yang Bo 2,4, Du Ying5, Hu Xiang3, Zheng Yi-duan3, Ning Xiao-lin3   

  1. 1 Postdoctoral Scientific Research Workstation of PKU-HKUST Shenzhen-Hong Kong Institution, Shenzhen  518057, Guangdong Province, China; 2 Clinical Medicine Postdoctoral Station of Zhengzhou University, Zhengzhou  450052, Henan Province, China; 3 Shenzhen Beike Cell Engineering Institute, Shenzhen  518057, Guangdong Province, China; 4 Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China; 5 Department of Microbiology and Immunology,College of Basic Medical Science, Zhengzhou University, Zhengzhou  450052, Henan Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Yang Bo, Professor, Clinical Medicine Postdoctoral Station of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China yangbo96@126.com
  • About author:Wu Shi-kai☆, Doctor, Postdoctoral Scientific Research Workstation of PKU-HKUST Shenzhen-Hong Kong Institution, Shenzhen 518057, Guangdong Province, China; Clinical Medicine Postdoctoral Station of Zhengzhou University, Zhengzhou 450052, Henan Province, China; Shenzhen Beike Cell Engineering Institute, Shenzhen 518057, Guangdong Province, China shikaiwu@gmail.com
  • Supported by:

    the Science and Technology Planning Project of Shenzhen, No. GC200806200138A*CJ200807210024A*; the Research Program of Combining Production with Education,Science and Technology Department of Guangdong Province, No. 2008B090500139*

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Abstract:

BACKGROUND: Many research pay attention to umbilical cord blood cells can inhibit tumor cells in recent years, people tried to suppress cancer cells by umbilical cord blood cell transplantation and these treatments may provide a new way for curing cancer.
OBJECTIVE: To investigate the effect of adhered mononuclear cells from human cord blood and gastric cancer cells after co-cultured in vitro.
METHODS: Cord blood mononuclear cells were separated by density gradient centrifugation, and the expression of surface marker of the mononuclear cells was detected by flow cytometry. Then the adhered cells and gastric cancer cells were co-cultured, and cell morphology were observed under an inverted microscope, and lactate dehydrogenase (LDH) detection was used to identify anti-tumor efficacy of cord blood mononuclear cells and to screen best effector/target ratio.
RESULTS AND CONCLUSION: At 2 days after accession of cord blood mononuclear cells, the shedding of tumor cells was obviously. In the experimental group, the tumor cells were arranged irregularly with low cell density. LDH method showed that some adherent cells from umbilical cord blood have strong anti-tumor capabilities, but the anti-tumor capacity in different individuals were varied in 4-fold. Adherent cells from umbilical cord blood have strong killing effect on tumor cells, and best anti-target ratio was 2:1.

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