Abstract:

BACKGROUND: The goal of in vitro amplification of umbilical cord hematopoietic cells is promoting the engrafting capacity of umbilical cord hematopoietic cells, and the cytokine mediating can accelerate cell amplification, which exhausted the hemopoietic stem cells simultaneously.   
OBJECTIVE: To explore the effect of human bone marrow stromal cells (BMSCs) in combination with several cytokines on the in vitro expansion of the mononuclear cells derived from umbilical cord blood and the expression of adhesion molecule and CXCR4 after cell expansion.
METHODS: The isolated human mononuclear cells were incubated on BMSCs layers and cultured in 4 groups. Cells in the control group were cultured in single mononuclear cells; those in the other groups were cultured in several cytokines + mononuclear cells, BMSCs+ mononuclear cells, BMSCs+ several cytokines, and several cytokines + mononuclear cells, respectively. At 0 and 7 days after culture, the number of total nucleated cells, CD34⁺ cells, CD34⁺CXCR4⁺ , CD34⁺CD49d⁺ , and CD34⁺CD62L⁺ cells were counted.
RESULTS AND CONCLUSION: The single BMSCs and cytokine could effectively promote the amplification of umbilical cord hematopoietic cells and increased the expressions of CD49d, CD62L and CXCR4. The cell multiplication ability in the single cytokine group was greater than that in the BMSCs group, but poorly increased the expressions of CD49d, CD62L and CXCR4. It revealed that BMSCs can support the amplification of umbilical cord hematopoietic cells, but the supporting ability is limited. However, BMSCs in combination with several cytokines can effectively promote amplification of umbilical cord hematopoietic cells, the results of which is superior to the single cytokine or BMSCs. The results demonstrated that BMSCs in combination with several cytokines can enhance expansion of cord blood mononuclear cells, and promote the adhesion and chemotactic capacity of umbilical cord hematopoietic cells.