Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (29): 5346-5350.doi: 10.3969/j.issn.1673-8225.2010.29.007

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Biocompatibility of hydroxyapatite surface modified titanium skirt for keratoprosthesis: Scanning electron microscopy observation

Li Zhao-hui1, Huang Yi-fei1, Ma Xiao1, Huang Jing-xiang2, Cui Fu-zhai3   

  1. 1 Department of Ophthalmology, General Hospital of China PLA, Beijing   100853, China; 2 Orthopaedics Institute, General Hospital of China PLA, Beijing   100853, China; 3 Department of Materials Science and Engineering, Tsinghua University, Beijing   100084, China
  • Online:2010-07-16 Published:2010-07-16
  • Contact: Huang Yi-fei, Professor, Doctor, Doctoral supervisor, Department of Ophthalmology, General Hospital of China PLA, Beijing 100853, China
  • About author:Li Zhao-hui★, Master, Associate chief physician, Department of Ophthalmology, General Hospital of China PLA, Beijing 100853, China zhaohuili650@hotmail.com
  • Supported by:

     Project of Chinese PLA During the 10th Five-Year Plan Period, No. 01MA109*, 06MA281*

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Abstract:

BACKGROUND: Keratoprosthesis surgery may be alternative in patients with corneal blindness of both eyes. Currently, researches for improving biocompatibility between the keratoprosthesis and the host tissue to achieve biological healing are predominant.
OBJECTIVE: To evaluate the biocompatibility of hydroxyapatite surface modified titanium skirt for keratoprosthesis by scanning electron microscopy.
METHODS: The bioactive hydroxyapatite coating was prepared on pure titanium for keratoprosthesis by an acid-alkali chemical pretreatment. The fibroblasts of rabbit cornea at 4 to 6 passages were directly seeded on hydroxyapatite modified titanium surfaces, pure titanium surfaces and glass surfaces. Cell adhesion, proliferation and morphology were detected at 3, 24, 48, 72 hours by scanning electron microscopy. A total of 18 normal New Zealand white rabbits were randomly divided into two groups. Skirt of hydroxyapatite modified titanium and pure titanium were implanted into the right eye corneal stroma of rabbits for 6 and 12 weeks, respectively. The growth and adhesion of the corneal tissue on the surface of skirt were examined by scanning electron microscopy.
RESULTS AND CONCLUSION: The in vitro experiment showed that, cells on hydroxyapatite modified titanium surfaces had greater spreading area and longer stress fibers than that on the pure titanium at 3 and 24 hours. Both hydroxyapatite modified titanium and pure titanium were superior to glass surface. The number of viable cells was the highest on hydroxyapatite modified titanium surfaces (P < 0.05). At 72 hours, hydroxyapatite modified titanium surfaces were totally covered by collagen. The in vivo experiment showed that, the extracellular matrix deposited well on the surface of hydroxyapatite modified titanium, tightly adhered with hydroxyapatite. The pure titanium was only covered with corneal tissues. Hydroxyapatite surface modification could improve the biocompatibility of pure titanium skirt for keratoprosthesis.

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