Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (24): 4371-4375.doi: 10.3969/j.issn.1673-8225.2010.24.001

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In vitro differentiation from bone marrow mesenchymal stem cells into chondrocytes induced by transforming growth factor-beta combined with bone morphogenetic protein-2

Zhang Qing-lin, Lü Hui-cheng, Wu Yi-min   

  1. Second Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical College, Huhehaote  010030, Inner Mongolia Autonomous Region, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Wu Yi-min, Doctor, Chief physician, Professor, Second Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical College, Huhehaote 010030, Inner Mongolia Autonomous Region, China
  • About author:Zhang Qing-lin★, Studying for master’s degree, Second Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical College, Huhehaote 010030, Inner Mongolia Autonomous Region, China linzi688@163.com

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Abstract:

BACKGROUND: Multiple studies have reported that transforming growth factor-β1 (TGF-β1) induced differentiation from bone marrow mesenchymal stem cells (BMSCs) into chondrocytes; however, reports addressing bone morphogenetic protein-2 (BMP-2) inducing the differentiation remain less.
OBJECTIVE: To verify the possibility and interaction of differentiation from BMSCs into chondrocytes induced by TGF-β1 and BMP-2.
METHODS: BMSCs were derived from healthy Wistar rats using adherent methods. The BMSCs were assigned into four groups: TGF-β1+BMP-2, TGF-β1, BMP-2, and control groups. At 14 days after induction, alcian blue staining and dimethyl-methylene blue chromatometry were employed to determine the glycosaminoglycan (GAG) level, and immuno-histochemistry was used to detect the expression of specific type II collagen.
RESULTS AND CONCLUSION: In the three experimental groups, alcian blue staining and type II collagen immunohistochemistry examinations showed that chondyocytes could express glycosaminoglycan (GAG) and type II collagen, and the GAG detection showed that the expression of GAG gene was significantly greater in the TGF-β1+BMP-2 group than in the TGF-β1 group and BMP-2 group (P < 0.05). TGF-β1 combined with BMP-2 was more effective for promoting the differentiation from BMSCs into chondrocytes, which might be used as the better source of the seed cells of cartilage tissue engineering.

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