Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (15): 2756-2760.doi: 10.3969/j.issn.1673-8225.2010.15.023

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Effects of in vitro pressure stimulation to fascia tissue of zusanli (ST36) on synthesis and release of fibroblast matrix metalloproteinase-1, matrix metalloproteinase inhibitor-1, prostaglandin E2 and insulin-like growth factor-1 

Chen Bo1, Cui Jin1, Xie Xi-mei1, Li Jia-lin1, Li Xiao-yu2   

  1. 1 Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China; 2 Key Laboratory of Oral Biomedical Engineering of Ministry of Education, West China College of Stomatology, Sichuan University, Chengdu  600041, Sichuan Province, China
  • Online:2010-04-09 Published:2010-04-09
  • About author:Chen Bo☆, Doctor, Associate professor, Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China chenbomed@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30801490*; Foundation of Science and Technology Commission of Guizhou Province, No. 2006[2063]*

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Abstract:

BACKGROUND: Fibroblasts in acupoint ascia tissues are major forces during acupuncture and massage therapy, and the regulation of cell biological behavior is closely associated with treatment efficiency.
OBJECTIVE: To observe the effect of in vitro pressure stimulation to zusanli (ST36) fascia on synthesis and release of fibroblast matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase inhibitor-1 (TIMP-1), prostaglandin E2 and insulin-like growth factor-1 (IGF-1).
METHODS: Fascia fibroblasts of zusanli (ST36) were cultured in vitro and randomly divided into the control and pressure groups. Cells in the pressure group were stimulated by 50 kPa initial pressure for 2 hours, with an 8-hour interval for 6 times. There were no pressures in the control group. The extracellular medium changes of MMP-1, TIMP-1, prostaglandin E2 and IGF-1 was detected, and the range MMP-1 and TIMP-1 ratio (MMP-1/TIMP-1) was calculated.
RESULTS AND CONCLUSION: After pressure stimulation on zusanli (ST36) fascia fibroblasts, the MMP-1, TIMP-1 and prostaglandin E2 levels were increased in culture medium (P < 0.05 or P < 0.01), MMP-1/TIMP-1 increased (P < 0.05), and the difference of IGF-1 had no statistically significance. The results demonstrated that, pressure stimulation on acupoints fascia fibroblasts has a positive regulation of MMP-1, TIMP-1 synthesis, as well as the improvement of MMP-1/TIMP-1. Maybe it is the mechanical cellular biological principle of acupoints acupuncture therapy of sujingtongmai.

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