Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (21): 3871-3876.doi: 10.3969/j.issn.1673-8225.2011.21.017

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Treatment of osteomyelitis in rabbits with plastic nanohydroxyapatite/poly (3-hydroxybutyrate-hydroxyvalerate)-polyethylene glycol drug delivery system

Zhang Bo-ping1, Tang Shan-hua1, Zhang Li1, Lü Ren-fa1, Lu Hai-feng1, Jin An-min2, Wang Xu-dong3   

  1. 1Department of Orthopaedics, No. 184 Hospital of PLA, Yingtan  335000, Jiangxi Province, China
    2Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
    3College of Biological Engineering and Materials Science, South China University of Technology, Guangzhou  510200, Guangdong Province, China
  • Received:2010-11-01 Revised:2010-12-10 Online:2011-05-21 Published:2011-05-21
  • Contact: Tang Shan-hua, Master, Associate chief physician, Department of Orthopaedics, No. 184 Hospital of PLA, Yingtan 335000, Jiangxi Province, China mouse_1001@163.com
  • About author:Zhang Bo-ping, Associate chief physician, Department of Orthopaedics, No. 184 Hospital of PLA, Yingtan 335000, Jiangxi Province, China lhf1010@qq.com

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Abstract:

BACKGROUND: Existing local drug delivery system is mostly massive, cannot arbitrary moulding and is difficult to completely sticking with the bone cavity, which easily lead to recurrence of osteomyelitis, and obvious effect of drug burst release, small amount of drug loading, short releasing time, and certain antigenicity. Therefore, a new type of plastic local drug delivery system was developed by task force and South China University of Technology, in order to provide a better method of treatment of osteomyelitis.
OBJECTIVE: To investigate the therapeutic effect of the plastic nanohydroxyapatite/poly (3-hydroxybutyrate-hydroxyvalerate)-polyethylene glycol-gentamicin local drug delivery system (nano-HA/PHBV-PEG-GM-DDS) on osteomyelitis.
METHODS: Proximal tibia osteomyelitis model of rabbits was prepared. Staphylococcus aureus was injected into proximal tibia bone window after 2 weeks and underwent debridement. The plastic nano-HA/PHBV-PEG-GM-DDS of 1 mL was implanted into groupⅠ, the plastic nano- HA/PHBV-PEG of 1 mL mixed gentamicin power of 23.2 mg into group Ⅱ, the plastic nano- HA/PHBV-PEG of 1 mL in conjunction with intramuscle gentamicin into group Ⅲ 5 days, a total of 23.2 mg, the plastic nano-HA/PHBV-PEG of 1 mL without antibiotic in group Ⅳ, and nothing into group Ⅴ. Specimens were harvestd 8 weeks after the above procedures and then were subjected to radiological, histological and bacteriological examinations.
RESULTS AND CONCLUSION: In groupⅠ, the bacteria counting and X-ray Norden scoring were by far the smallest among all 5 groups (P < 0.01)，with no histological manifestation of osteomyelitis. In groupⅡ, Ⅲ, and Ⅳ, the bacteria counting gradually increased and there was significant difference among three groups (P < 0.01). However, there was no significant difference in improved X-ray Norden scoring among 3 groups (P > 0.05); histological manifestation had also no essential difference. The bacteria counting and Norden scoring as well as the histological manifestation in groupⅡ, Ⅲ, and Ⅳ were significantly higher than those in group Ⅴ (P < 0.01 or 0.05), its histology showed severe osteomyelitis manifestation. The results showed that the plastic nano- HA/PHBV-PEG-GM-DDS could be implanted as primary graft into the remaining infected defect after debridement to effectively treat osteomyelitis. Conventional systemic antibiotic or simple local antibiotic following debridement was not effective in treating chronic osteomyelitis. Primary bone grafting would rather make the condition worse, under without any antibiotic or conventional systemic antibiotic or simple local antibiotic.

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